脉冲场凝胶电泳在肠炎沙门菌食源性疾病溯源中的应用

目的对2006年广州地区食源性疾病中分离的肠炎沙门菌进行分子分型,探讨广州地区肠炎沙门菌的分子型别和多态性,为食源性疾病溯源及致病菌数据库的建立提供依据。方法采用限制性内切酶XbaI,对2006年分离到的菌株进行PFGE分子分型,使用BioNumericsVersion4.0软件(使用Dice系数和UPGMA法)对菌株进行聚类分析,并与深圳市的肠炎沙门菌PFGE型别进行比较。结果所有74株肠炎沙门菌均得到一致的PFGE克隆型,表明两次不同的食源性疾病均由同一PFGE型引起。广州与深圳的肠炎沙门菌PFGE图谱的比较表明,两地食源性疾病分离株具有很近的亲缘关系。结论PFGE分子分型与流行病学资料紧密结合可增强对肠炎沙门菌食源性疾病的溯源和预警。

Application of pulse-field gel electrophoresis in the source-tracking of food-borne diseases caused by Salmonella enteritidis

To subtype molecularly Salmonella enteritidis(S.enteritidis)isolated from food-borne diseases and to explore the molecular subtypes and polymorphism of S.enteritidis in Guangzhou aera in order to provide data for source-tracking of food-borne diseases and establishment database of pathogenic bacteria.Pulse-field gel electrophoresis(PFGE)using restriction enzyme Xba I was employed for the molecular subtyping of S.enteritidis isolated in 2006 and PFGE patterns were analyzed by BioNumerics Version 4.0 software to perform cluster analysis.Pattern profiles were compared by utilizing Dice coefficient and UPGMA(unweighted pair group method with arithmetic averages).The pulsotype of S.enteritidis isolated in Guangzhou aera was compared with that of S.enteritidis in Shenzhen.All the 74 strains of S.enteritidis shared identical pulsotype,indicating that the two independent events of food-borne diseases were triggered by the same pulsotype.Additionally,comparison of pulsotypes of S.enteritidis strains isolated in Guangzhou and Shenzhen showed that strains from the two cities have very close relationship.It is evident that PFGE subtyping,coupled with epidemiological data,can enhance source-tracking of and warning against food-borne diseases caused by S.enteritidis.