UPLC法测定保健品中五种人参皂苷的含量

目的:建立人参类保健品中五种人参皂苷Rg1、Re、Rb1、Rc、Rd的超高效液相色谱分析方法。方法:样品经甲醇提取,大孔树脂柱净化,进行色谱分析。色谱条件:ACQUITY UPLC BEH C18色谱柱(2.1×50 mm,1.7μm);乙腈-水流动相体系;紫外检测,检测波长为203 nm;柱温30℃;流速为0.50 ml/min。结果:五种人参皂苷在20 min内得到良好分离,方法的线性范围为39.6μg/ml～500.0μg/ml,线性相关系数为0.9995～0.9997。最小检出量为0.8 ng～2.5 ng,回收率为78.6%～94.4%,相对标准偏差2.4%～6.4%。结论:本法具有简单、快速、准确、可靠的特点,能够满足日常分析检测的需要,为人参类保健食品的质量控制提供了简便方法。

Determination of five ginsenosides in health products by UPLC

Objective:To develop a method for the determination of five ginsenosides(Rg1,Re,Rb1,Rc,Rd) in ginseng health products by UPLC.Methods: Samples were extracted by methanol,purified through macroporous adsorbents,and then the separation was performed on ACQUITY UPLC BEH C18 column(2.1×50 mm,1.7 μm)set at 30℃ for gradient elution,using the solution of acetonitrile and water as mobile phase at the flow rate of 0.50 ml/min and detected at 203 nm.Results: Five ginsenosides were well separated within 20 min.The calibration curves of ginsenosides showed good linearity in 39.6 μg/ml～500.0 μg/ml with correlation coefficients of 0.9995～0.9997.The detection limits of ginsenosides were 0.8 ng～ 2.5 ng.The recovery ranges were 78.6%～94.4%,and the RSDs were 2.4%～6.4%.Conclusion: The method is simple,rapid,sensitive and accurate,and suitable for the detection of five ginsenosides in health products.