外科重症监护室分离MRSA的流行病学研究

目的了解某院外科重症监护室(SICU)患者分离的耐甲氧西林金黄色葡萄球菌(MRSA)与同期环境分离的MRSA的同源性,为控制MRSA的院内传播提供依据。方法对2010年8—10月SICU送检临床标本进行MRSA的分离鉴定,同时对环境进行采样检测MRSA。对分离到的MRSA菌株,应用基于细菌基因组重复序列聚合酶链反应(Rep-PCR)技术的DiversiLab系统进行同源性分析。结果 124份临床标本共分离金黄色葡萄球菌12株,经鉴定全部为MRSA。对其中10株MRSA和环境分离的3株MRSA进行同源性分析,发现9株患者来源的MRSA和3株环境来源的MRSA具有高度同源性;另1株患者来源的MRSA不同于环境来源的MRSA。结论 SICU患者分离的MRSA与环境分离的MRSA呈高度同源性,可能为同一克隆株。该SICU MRSA的院内传播较为严重。

Epidemiological study on methicillin resistant Staphylococcus aureus isolated from an intensive care unit

Objective To investigate the DNA homology of MRSA isolated from patients in a surgical intensive care unit（SICU） and SICU environment,so as to provide reference for the control of MRSA spread in hospital.Methods MRSA were isolated from SICU patients and environment from August to October 2010.Rep-PCR based DiversiLab microbial typing system was applied to analyze the homology of MRSA.Results Of 124 samples,12 MRSA were isolated,with 9 of 10 MRSA isolates from patients and 3 MRSA isolates from environment.They were highly homologous.Conclusion MRSA isolated from SICU patients are highly homologous to those from SICU environment.MRSA transmission in this SICU is serious.