铜绿假单胞菌医院感染与环境因素相关性研究

目的了解铜绿假单胞菌(PA)在医院感染及环境监测中的分布,探讨二者的关系。方法按常规方法对某院2006年6月-2009年6月各类临床标本进行细菌培养与分离,采用VITEK32全自动微生物鉴定仪进行菌株鉴定。PA血清分型采用日本生研株氏会社分型血清进行。结果196株临床分离的PA血清分型率为90.82%（178株），以G（26.02%）、E（15.31%）、F（14.29%）、B（10.21%）、H（9.69%）、I（7.14%）型为主，未发现的血清型有A、J和K型；25株环境分离的PA血清分型率为96.00%（24株），以G（32.00%）、F（24.00%）、B（16.00%）、E（12.00%）型为主。结论从患者标本与环境中分离的PA菌株具有相似的血清型，表明其具有一定相关性；菌株血清分型结果可在一定条件下将细菌检验工作与医院感染监测工作有效地结合起来, 用于临床流行病学调查。

Relation between Pseudomonas aeruginosa healthcare associated infection and environmental factor

Objective To understand the distribution of Pseudomonas aeruginosa（PA） in healthcare-associated infection（HAI） as well as the environment,and to evaluate their relation.Methods Bacteria in clinical samples from a hospital from June,2006 to June,2009 were cultured and isolated with routine method,and strains were identified by using VITEK32 system.Serotyping of PA were performed by using standard serum of PA.Results Of 196 clinically isolated PA,serotyping rate was 90.82%（178 isolates）,the main serotypes were type G（26.02%）,E（15.31%）,F（14.29%）,B（10.21%）,H（9.69%）,and I（7.14%）,but type A,J,and K were not found;Of 25 environmentally isolated PA,serotyping rate was 96.00%（24 isolates）,the main serotypes were type G（32.00%）,F（24.00%）,B（16.00%）,and E（12.00%）.Conclusion PA isolated from patients and environment has similar serotypes,it shows that there is certain relation;Bacterial serotyping can combine bacterial detection with HAI surveillance,and can be used for clinical epidemiological investigation.