FISH技术检测宫颈组织TERC基因扩增

目的应用荧光原位杂交技术（FISH）检测子宫颈病变组织中人端粒酶RNA（TERC）基因异常扩增的临床意义。方法应用双色荧光原位杂交技术（FISH）检测195例宫颈组织TERC基因的异常扩增。结果①195例各类宫颈病变中,慢性宫颈炎33例,ClN134例,CIN2/3（包括原位癌）37例,宫颈鳞状细胞癌30例,宫颈腺癌61例,用FISH检测TERC基因的阳性表达率分别是3.03%（1/33）、29.41%（10/34）、72.97%（27/37）、100%（30/30）、91.80%（56/61）,子宫颈鳞癌与腺癌TERC基因的表达较宫颈上皮内瘤变各组差异有统计学意义（P〈0.05）。TERC基因的扩增阳性率随着宫颈病变程度增加呈逐渐上升趋势,且TERC扩增在子宫颈鳞癌与腺癌患者中无明显差异性;②TERC基因异常扩增与高危型HPV感染呈正相关。结论应用FISH技术检测TERC基因的异常扩增可以作为组织学诊断困难的病变确诊、病变预测及治疗后风险评估的手段。

Expression of the human telomerase gene in cervical tissue detected with FISH

Objective To detect the human telomerase gene (TERC) amplification by dualcol or interphase DNA probe fluorescence in situ hybridization ( FISH ) in cervical carcinoma and CIN.Methods 195cases of cervical lesion were detected the TERC expression by FISH.Results Among 195cases , the positive rate of TERC gene in chronic cervicitis , CIN1 , CIN2/3 , cervical squamous cell carcinoma , cervica1 adenocarcinoma was 3.03% (1/33), 29.41% (10/34), 72.97% (27/37), 100% (30/30) and 91.80% (56/61), respectively ; the difference between cervical carcinoma and chronic cervicitis and CIN groups was significant (P<0.05) .The positive rate of TERC gene amplification increasing with the grade of CIN increased ; but it has no difference between cervical squamous cell carcinoma and cervica1 adenoeareinoma.Conclusion The detection of amplification of TERC by FISH can assist to identifying cervical lesions with high sensitivity and specificity.