氟伐他汀对糖尿病大鼠心肌单核细胞趋化因子-1表达的影响

目的探讨氟伐他汀对糖尿病大鼠心肌单核细胞趋化因子-1(MCP-1)表达的影响及意义。方法 32只大鼠分为正常对照组、糖尿病未治疗组、氟伐他汀小剂量治疗组及氟伐他汀大剂量治疗组,12周后称质量并计算心脏质量体质量比(H/B)和左室质量指数(LVMI),Western blot检测各组心肌MCP-1、纤维连接蛋白(FN)、Ⅲ型胶原(ColⅢ)的表达。结果糖尿病未治疗组H/B、LVMI增高;MCP-1、FN和ColⅢ的表达显著上调。氟伐他汀治疗能降低H/B(P<0.05～0.01)及LVMI(P<0.01);减少糖尿病大鼠心肌MCP-1、FN和ColⅢ的表达(均P<0.01),以上效应具有剂量依赖性。结论氟伐他汀可抑制糖尿病大鼠心肌细胞外基质积聚,MCP-1表达下调可能是其心脏保护的作用机制之一。

Effect of Fluvastatin on Expression of Monocyte Chemotactic Factor-1 in the Myocardium of Diabetic Rats

Objective To investigate the effect s of fluvastatin on expression of monocyte chemotactic factor-1(MCP-1) in the myocardium of diabetic rats.Methods Thirty-two Sprague-Dawley rats were randomized into four groups : normal control,untreated and STZ-induced diabetic rats,and diabetic rats treated with low-dose and high-dose fluvastatin.The ratio of heart weight to body weight(H / B) and the left ventricle mass index(LVMI) was calculated and the expression of MCP-1,Fibronectin(FN),CollagenⅢ(ColⅢ) in the myocardium was determined by western blot after 12weeks intervention.Results Compared with the normal control group,the untreated diabetic group showed the increased H / B,LVMI and expression of MCP-1,FN,ColⅢin the myocardium(all P<0.01).Fluvastatin treatment could significantly reduce the increment of H / B and LVMI(P<0.05～0.01),suppress expression of MCP1,FN,ColⅢin the myocardium of diabetic rats(P<0.01).All these effects were in dose dependent way.Conclusions Fluvastatin treatment inhibits extracellular matrix(ECM) accumulation in the myocardium of diabetic rat,and the down-regulation of MCP-1in diabetics may be an underlying mechanism of fluvastatin in the cardiac protection.