降钙素基因相关肽和神经生长因子对短暂性全脑缺血后再灌注大鼠纹皮质c-jun mRNA及蛋白表达的影响

目的探讨外源性降钙素基因相关肽（CGRP）和神经生长因子（NGF）对短暂性全脑缺血后再灌注大鼠纹皮质c-jun mRNA及蛋白表达的影响。方法原位杂交和免疫组织化学结合显微图像分析方法。结果假手术组大鼠纹皮质c-jun mRNA表达弱；缺血组较假手术组c-jun mRNA表达显著强（P〈0．01）；CGRP组和NGF组c-jun mRNA表达弱于缺血组（P〈0．05）；CGRP和NGF合用组c-jun mRNA表达明显弱于缺血组（P〈0．01），分别弱于CGRP组和NGF组（P〈0．05）。假手术组大鼠纹皮质未见c-Jun蛋白表达；缺血组较假手术组c-Jun蛋白表达明显强（P〈0．01），缺血后再灌注3h强，1d、3d时弱；CGRP组和NGF组c-Jun蛋白表达较缺血组弱（P〈0．05）；CGRP和NGF合用组较缺血组c-Jun蛋白表达明显弱（P〈0．01），分别弱于CGRP组和NGF组（P〈0．05）；CGRP和NGF合用组缺血后再灌注3h时强，1d、3d时弱。结论CGRP和NGF分别抑制全脑缺血后再灌注大鼠纹皮质c-jun mRNA及蛋白表达，联合应用显著抑制全脑缺血后再灌注大鼠纹皮质c-jun mRNA及蛋白表达，两者对保护缺血神经元可能有协同作用。

THE EFFECT OF CALCITONIN GENE RELATED PEPTIDE AND NERVE GROWTH FACTOR ON THE EXPRESSION OF c-jun mRNA AND PROTEIN IN STRIATE CORTEX OF RATS WITH TRANSIENT GLOBAL CEREBRAL ISCHEMIA/REPERFUSION

Objective To study the effects of calcitonin gene related peptide(CGRP) and nerve growth factor(NGF) on the expression of(c-junmRNA) and protein in striate cortex of rats with transient global cerebral ischmia/reperfusion. Methods In situ hybridization,immunohistochemistry and microscope image analysis were used. Results The expression of c-jun mRNA in ischemia/reperfusion(I/R) group was increased as compared with sham group(P<0.01).The expression of c-jun mRNA was lower in NGF group than that in I/R group(P<0.05),and lower in CGRP group than that in I/R group(P<0.05),and markedly lower in NGF and CGRP group than that I/R group(P<0.01),and in NGF and CGRP group lower than that in NGF group and CGRP group,respectively(P<0.05).The expression of c-Jun protein in I/R group was increased as compared with sham group(P<0.01).The expression of c-Jun was lower in NGF group than that in I/R group(P<0.05),and lower in CGRP group than that in I/R group(P<0.05),and markedly lower in NGF and CGRP group than that in I/R group(P<0.01),and lower in NGF and CGRP grop than that in NGF group and CGRP group respectively(P<0.05).The expression of c-Jun in I/R group was stronger(3?h) survival after ischemia/reperfusion than 1d survival and 3d survival(P<0.05),and stronger in NGF and CGRP group 3h survival after ischemia/reperfusion than 1d survival and 3d survival(P<0.05).Conclusion The CGRP and NGF downregulate the expression of(c-jun) mRNA and protein,and combined use of NGF and CGRP can markedly downregulate the expression of(c-jun) mRNA and protein in striate cortex of global cerebral ischemia rats.The present results indicate that CGRP and NGF might have coordinate protective effects on the ischemia neurons in striate cortex.