新西兰兔烫伤模型的建立

目的：建立一种简易的可控制面积及深度的烫伤模型．方法：以医用纱布作为热载体，确定可持续保温的最佳纱布层数．将恒温热水浴中加热至99℃的纱布分别在新西兰兔背部烫3，5，7，9，11，13，15，17，19，21，23，25s共计12个时间点．伤后24h取标本，经HE染色评价烫伤程度，并埘烫伤的时间和程度作相关性分析．结果：确定可持续保温的纱布最佳层数为35层，并且烫伤程度和时间具有很好的相关性：Ⅰ度烫伤模型的致伤时间3—5S；浅Ⅱ度烫伤模型的致伤时间7—13s；深Ⅱ度烫伤模型的致伤时间15～19S；Ⅲ度烫伤模型的致伤时间21—25s．结论：该模型选用纱布作为热载体，具有烫伤面积及深度控制准确，操作简便、重复性好的优点，是研究创伤修复机制的较好模型．

Establishment of scalding model of New Zealand rabbit

AIM： To establish a simple scalding inodel with controllable area and grades. METHODS： Pledgets were used as heating media. At first, the optimal layer numbers of pledgets were determined by evaluating the effect of heat preservation. Pledgers soaked with hot water at 99 ℃ were applied onto the backs of New Zealand rabbits for 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 and 25 s respectively. After 24 h, samples were harvested and the scalding lesions were evaluated by HE staining. Also, the relationship between the heating time and scalding grades were statistically analyzed. RESULTS： The optimal layer numbers of pledgers proved to be 35. Importantly, there was a close relationship between the heating time and scalding grades. The time for preparing Ⅰ , superficial Ⅱ, deep Ⅱ and Ⅲ grades scald was3-5, 7- 13, 15- 19 and 21 -25 s respectively. CONCLUSION： The current scalding model induced by pledgets as heating media boasts advantage of controllable areas and grades, easy manipulation and good reproducibility, which may be a valuable model to investigate the mechanism of repair in trauma.