| PI3K/AKT特异性抑制剂LY294002对胆管癌细胞化疗增敏作用的研究 |
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| 引用本文： | 刘民锋,余险峰,郭伟,董泾青,左石,罗剑,徐立宁,邹声泉.PI3K/AKT特异性抑制剂LY294002对胆管癌细胞化疗增敏作用的研究[J].山东医药,2006,46(8):6-7. |
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| 作者姓名： | 刘民锋 余险峰 郭伟 董泾青 左石 罗剑 徐立宁 邹声泉 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院,湖北武汉430022 |
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| 摘 要： | 目的探讨磷脂酰肌醇-3-激酶抑制剂（PI3K）LY29400222（42吗啉基）282苯基24氢212苯并吡喃242酮]对胆管癌化疗效果的影响。方法将LY294002用于胆管癌细胞系QBC939．MTT法检测单独使用5-Fu、MMC、塞莱西布及联合磷酸化抑制剂LY294002，对体外培养的胆管癌细胞系QBC939的抑制作用；流式细胞技术检测QBC939凋亡抑制率。结果LY294002作用后．5-Fu、MMC及塞莱西布对胆管癌细胞系QBC939抑制作用明显增强．且能提高其凋亡率。结论LY294002能有效提高化疗药物5-Fu、MMC、塞莱西布体外对QBC939细胞抑制作用的敏感性；抑制PI3K介导的信号转导通路，可明显提高胆管癌的化疗效果。
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| 关 键 词： | 信号转导通路 胆管肿瘤 细胞凋亡 |
| 文章编号： | 1002-266X（2006）08-0006-02 |
| 收稿时间： | 2005-11-02 |
| 修稿时间： | 2005年11月2日 |
Effect of PI3K/AKT inhibitor LY294002 on increasing sensitivity of regular chemotherapeutic agent of cholangiocarcinoma |
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| Liu MinFeng;Yu XianFeng;Guo Wei;Dong JingQing;Zuo Dan;Luo Jian;Xu LiNing;Zou ShengQuan.Effect of PI3K/AKT inhibitor LY294002 on increasing sensitivity of regular chemotherapeutic agent of cholangiocarcinoma[J].Shandong Medical Journal,2006,46(8):6-7. |
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| Authors: | Liu MinFeng;Yu XianFeng;Guo Wei;Dong JingQing;Zuo Dan;Luo Jian;Xu LiNing;Zou ShengQuan |
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| Abstract: | Objective: To explore the effect of PI_3K/AKT inhibitor LY294002 on increasing sensitivity of regular chemotherapeutic agent of cholangiocarcinoma.Methods: By using MTT detected the inhibition of cholangiocarcinoma when singly applied with 5-Fu,MMC,CELECOXIB contrast to associated with LY294002.At the same condition,detect the apoptosis ratio by the flow cytometer.Result: LY294002 could increase the sensitivity of the 5-Fu,MMC,CELECOXIB and increase the apoptosis ratio in vitro.Conclusion: The LY294002 can enhance significantly the sensitivity of the cyto-inhibition of the chemotherapeutic agents.Inhibiting the PI_3K/AKT signaling pathway can increase the chemotherapeutic effect of cholangiocarcinoma. |
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| Keywords: | LY294002 |
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