北京地区人群中人Boca病毒血清抗体的分析

目的 通过对血清中人Boca病毒(HBoV)主要衣壳蛋白VP2特异性IgG抗体进行检测,初步了解北京地区人群中这种新发现的病毒的感染状况.方法 以大肠杆菌表达的HBoV主要衣壳蛋白VP2为抗原,应用Western-blot方法,对1996年4月至1997年3月取自首都儿科研究所附属儿童医院健康查体者及北京宣武医院非呼吸道感染患者的血清标本共677份进行HBoV VP2蛋白特异性IgG抗体检测.设抗组氨酸抗体及兔抗HBoV-VP2多肽特异性免疫血清为阳性血清对照.结果 (1)677份血清标本中,抗HBoV VP2蛋白的IgG抗体阳性400份,总检出率为59.1%.(2)被检对象中,＜1个月的婴儿抗体阳性率为45.3%,1个月～的婴儿抗体阳性率为41.4%,2个月～的婴儿抗体阳性率最低(31.3%),6个月～至7岁龄抗体阳性检出率在45.6%～69.7%,7岁后直至40岁,抗体阳性检出率维持在70%左右;50岁后则为61.8%～62.8%.结论 早在1996年北京地区的人群中就有59.1%曾经感染过HBoV,说明这种病毒是一种新发现的病毒而不是新出现的病毒,北京地区人群中该病毒的感染较常见.6个月龄以前的婴儿为易感人群.

Seroprevalence of antibody against human bocavirus in Beijing, China

Objective To find out the importance of human bocavirus(HBoV)as an infectious agent for population in Beijing,China,seroprevalence study was conducted by using expressed recombinant major capsid VP2 protein as an antigen.Methods Serum specimens collected from infants and children who visited the Children's Hospital Affiliated to the Capital Institute of Pediatrics for health check up and adults visited the Xuanwu Hospital,Beijing for diseases other than respiratory infections from April 1996 to March 1997 were used for investigation.The major capsid protein VP2 from HBoV was expressed in E.coli strain BL21(DE3)with the transformed PET30b vector inserted with full-length VP2 gene of HBo V and the specific antigenicity of this expressed protein was validated by previous study.Western blot was used to detect specific IgG antibody against HBoV in collected serum specimens diluted to 1:200.Mock expressed protein was E.coli cells strain B121(DE3)with the transformed PET30b vector without insert.Anti-His monoclonal antibody and rabbit anti-HBoV VP2 polypeptides hyper-immune serum were used as positive control for antibody detection.Results Out of 677 serum specimens tested,400(59.1%)were positive by Western blot.About 45.3%(34/75)of the newborns under 1 month of age had anti-HBoV antibodies,and antibody positive rates were lower in the age groups of 1 and 2 months(41.4% and 31.3%,respectively)and were higher in the following ages from 6 months to 7 years(from 45.6% to 69.7%).The antibody positive rates were at a relatively constant level(about 70%)in the age groups from 7 years to 40 years and became lower(61.8%-62.8%)in groups of age over 50 years.Conclusion The high seroprevalence against recombinant HBoV VP2 protein and early age antibody acquisition indicate that HBoV has been circulating in Beijing,China as early as in 1996 and most of children had been exposed to HBoV by the age of 7 years.Infants under the age of 6 months were susceptible to infection with this virus.