| 丹参酚酸B苦参碱复盐片中丹参酚酸B的含量测定研究 |
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| 引用本文： | 高红英,王航宇,李国玉,张珂,卢禁,谢石安,王金辉. 丹参酚酸B苦参碱复盐片中丹参酚酸B的含量测定研究[J]. 农垦医学, 2010, 32(2): 114-117 |
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| 作者姓名： | 高红英 王航宇 李国玉 张珂 卢禁 谢石安 王金辉 |
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| 作者单位： | 1. 石河子大学药学院药理学2007级,新疆石河子,832002
2. 石河子大学药学院教育部省部共建新疆特种植物药资源重点实验室,新疆石河子,832002 |
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| 摘 要： | 目的：建立测定丹参酚酸B苦参碱复盐片中丹参酚酸B含量的HPLC法。方法：采用Scienhome C18柱（4.6mm×250mm,5μm）,流动相为乙腈-0.3%磷酸水溶液梯度洗脱,流速1.0ml/min,检测波长286nm,进样体积10μl。结果：丹参酚酸B在0.0188～0.1696μg/μl范围内呈良好的线性关系,Y=1206930.2X-939.7,r=1,精密度RSD0.56%,重复性RSD1.4%,平均回收率在99.75%,RSD为0.99%。结论：该方法简单、结果准确、重现性好,适合于丹参酚酸B苦参碱复盐片在工业化生产和研究过程中的质量监控。
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| 关 键 词： | 高效液相色谱（HPLC）法 丹参酚酸B 含量测定 |
Determination of Salvianolic Acid B in the Matrine Salvianolic Acid B Salt Tablets by HPLC Method |
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| GAO Hong-ying,WANG Hang-yu,LI Guo-yu,ZHANG Ke,LU Jin,XIE Shi-an,WANG Jin-hui. Determination of Salvianolic Acid B in the Matrine Salvianolic Acid B Salt Tablets by HPLC Method[J]. Agricultural Reclamation Medicine, 2010, 32(2): 114-117 |
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| Authors: | GAO Hong-ying WANG Hang-yu LI Guo-yu ZHANG Ke LU Jin XIE Shi-an WANG Jin-hui |
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| Affiliation: | 1.Pharmaclogy,Post-graduate of Shihezi University(2007); 2.Key Laboratory of PhytomedicineResources ＆ Modernization of TCM in Pharmacy of Shihezi University;Xinjiang Shihezi,832002) |
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| Abstract: | Objective：To establish an HPLC method for the determination of salvianolic acid B in the Matrine Salvianolic acid B Salt Tablets.Methods：The Scienhome C18 （4.6mm×250mm,5μm） column was used. The mobile phase was consisted of acetonitrile-0.3% phosphoic acid with gradient elution. The detection wavelength was 286 nm.Results：The calibration curve of salvianolic acid B was liner in the range of 0.0188~0.1696 μg/μl （r = 1） with average recovery 99.75 % （0.99%）. Conclusion：The determination method was accurate,stable and could be used to control the quality of Matrine Salvianolic acid B Salt Tablets. |
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| Keywords: | HPLC Salvianolic acid B Determination |
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