一种碘伏消毒液的杀菌效果与毒性试验观察

目的了解一种碘伏消毒液的杀菌效果及其毒性。方法用悬液定量杀菌试验和动物试验方法对该碘伏消毒液进行了杀菌效果和毒性观察。结果用含有效碘250 mg/L的该碘伏消毒液作用1 min,对悬液内金黄色葡萄球菌平均杀灭对数值为5.55,对绿脓杆菌平均杀灭对数值为5.7;对白色念珠菌作用2 min,杀灭对数值为6.37。用含有效碘250 mg/L的该碘伏消毒液擦拭消毒作用2 min,对志愿者前臂皮肤表面自然菌的杀灭对数值均为1.00以上。该碘伏消毒液对小鼠急性经口LD50大于5000 mg/?体重,属实际无毒;该碘伏消毒液原液对试验动物完整皮肤和眼黏膜无刺激性;对小鼠骨髓嗜多染红细胞未发现微核作用。结论该碘伏消毒液具有良好的杀菌效果、毒性低、无刺激性、无致微核作用。

OBSERVATION ON GERMICIDAL EFFICACY AND TOXICITY OF A POVIDONE-IODINE DISINFECTION SOLUTION

Objective To explore the germicidal efficacy and toxicity of a povidone-iodine disinfection solution.Methods Suspension quantitative germicidal test and animal toxicity test were used to observe the germicidal efficacy and toxicity of the povidone-iodine disinfection solution.Results The average killing logarithm value of Staphylococcus aureus in suspension exposed to povidone-iodine disinfection solution containing available iodine 250 mg/L for 1 min was 5.55,that of Pseudomonas aeruginosa exposed for 1 min was 5.70 and that of Candida albicans exposed for 2 min was 6.37.The killing logarithm values of natural bacteria on forearm skin surface of volunteer exposed to this disinfection solution containing available iodine 250 mg/L for 2 min were >1.00.The acute oral LD50 of the povidone-iodine disinfection solution in mice was >5000 mg/kg·bw,which belonged to nontoxic class.No irritant effect on skin and eye of experimental animals was observed.It could not cause micronucleus formation in mouse bone marrow polychromatic erythrocytes.Conclusion This povidone-iodine disinfection solution has good germicidal efficacy,low toxicity and no irritating action,and does not cause micronucleus formation.