| 巨噬细胞NO_2~－释放法检测小鼠IFN－γ |
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| 引用本文： | 倪若愚,郭劲松,罗端德.巨噬细胞NO_2~－释放法检测小鼠IFN－γ[J].现代免疫学,1995(2). |
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| 作者姓名： | 倪若愚 郭劲松 罗端德 |
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| 作者单位： | 同济医科大学附属协和医院 |
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| 摘 要： | 本文建立了一种新的ＩＦＮ－γ检测方法。此方法利用ＩＦＮ－γ能够诱导ＭΦ产生并释放ＮＯ的原理，用微量显色技术对其衍生物亚硝酸盐ＮＯ进行定量测定。结果表明，样品中ＩＦＮ－γ的含量与ＭΦ释放ＮＯ的量有很好的相关性，抑制试验证实这种方法具有特异性。与ＩＦＮ－γ一起加入少量ＬＰＳ可以提高方法的敏感性和特异性。其它细胞因子，如ＩＬ－１、ＩＬ－２、ＴＮＦ、ＩＦＮ－β，对ＮＯ释放量的影响较小，可以忽略不计。此方法可供检测实验动物的ＩＦＮ－γ之用，有较高实用价值。
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| 关 键 词： | IFN－γ，NO_2~－释放，检测 |
The NO_2￣- Releasing Assay in Macrophages for the Detection of Murine IFN-γ |
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| Ni Ruoyu, Guo Jinshong, Luo Duande.The NO_2￣- Releasing Assay in Macrophages for the Detection of Murine IFN-γ[J].Current Immunology,1995(2). |
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| Authors: | Ni Ruoyu Guo Jinshong Luo Duande |
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| Abstract: | We have developed a new method for the detection of IFN-γ. In the experiment,peritoneal macrophage was isolated from Balb/c mice and was cultured for 48h with IFN-γ in presence of 10ng/ml LPS. NO was released to the culture fluids by the macrophage due to IFN-γ stimulation. The concentration of NO was determined by a simple micro-colorimetric assay, which was correlated to the amount of IFN-γ and was inhibited by antibody to IFN-γ. The influences by several other cytokines were minimal. This method is a simple and reliable assay to quantitate murine IFN-γ. |
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| Keywords: | IFN-γNO_2￣- detection |
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