| 神经节苷脂在大鼠脑外伤中对GAP-43蛋白表达的影响 |
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| 引用本文： | 李威,周军,廖达光,刘峰,韩永丰.神经节苷脂在大鼠脑外伤中对GAP-43蛋白表达的影响[J].现代医药卫生,2011,27(11):1601-1603. |
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| 作者姓名： | 李威 周军 廖达光 刘峰 韩永丰 |
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| 作者单位： | 中南大学湘雅三医院神经外科,湖南,长沙,410013 |
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| 摘 要： | 目的:研究神经节苷脂对大鼠自由落体脑损伤后脑内生长相关蛋白(GAP-43)表达的影响.方法:将S-D雄性大鼠随机分为4组:即正常对照组,假手术组,脑损伤(TBI)模型组,TBI后GM-1治疗组.复制大鼠自由落体脑损伤模型,在外伤后1、3、7、14 d,利用HE染色观察神经细胞形态和免疫组化染色检测神经细胞内GAP-43蛋白表达的变化.结果:脑内GAP-43蛋白的表达水平在头部损伤后逐渐升高,以伤后第三天、第七天最明显,随后有所下降,至第十四天仍轻度增高,TBI组各时段的GAP-43阳性蛋白表达均高于同时段正常对照组及假手术组,差异有统计学意义;神经节苷脂治疗组能促进TBI后GAP-43蛋白的表达,与同时段模型组对比差异有统计学意义,P均<0.05.结论:GAP-43蛋白在大鼠受到脑外伤后表达增高,可促进中枢神经损伤后的再生.神经节苷脂GM-1在TBI后可促进脑内GAP-43的表达,有稳定细胞膜、减轻细胞水肿、促进神经再生的作用.
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| 关 键 词： | 神经节苷脂 神经再生 GAP-43蛋白 免疫组化染色 |
Effects of GM-1 on GAP-43 protein expression in rat brain after traumatic brain injury |
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| LI Wei
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ZHOU Jun
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LIAO Da-guang
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LIU Feng
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HAN Yong-feng.Effects of GM-1 on GAP-43 protein expression in rat brain after traumatic brain injury[J].Modern Medicine Health,2011,27(11):1601-1603. |
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| Authors: | LI Wei ZHOU Jun LIAO Da-guang LIU Feng HAN Yong-feng |
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| Institution: | LI Wei,ZHOU Jun,LIAO Da-guang,LIU Feng,HAN Yong-feng(Department of Neurosurgery,Third Xiangya Hospital of Central South University,Changsha,Hunan 410013,China) |
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| Abstract: | Objective:To observe the effects of ganglioside(GM-1) on GAP-43 protein expression in rat brain with traumatic brain injury(TBI) caused by free-falling body.Methods:SD rats were randomly divided into 4 groups,normal control group,sham-operation group,TBI model group and GM-1 treatment group.The HE and immunochemical methods were used to detect the nerve cell morphology and the expression of GAP-43 protein at several time intervals after injury.Results:GAP-43 protein expression was increased gradually on 3 d... |
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| Keywords: | GM-1 Nerve regeneration GAP-43 protein Immunochemical staining |
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