| Survivin基因在急性胰腺炎中的表达及其意义 |
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| 引用本文： | 李震东,马清涌,罗羽宏.Survivin基因在急性胰腺炎中的表达及其意义[J].南方医科大学学报,2009,29(6):1141. |
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| 作者姓名： | 李震东 马清涌 罗羽宏 |
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| 作者单位： | 1. 暨南大学附属第一医院肝胆外科,广东,广州,510630
2. 西安交通大学第一医院肝胆外科,陕西,西安,710061 |
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| 摘 要： | 目的 探讨survivin基因在鼠急性胰腺炎中的表达及其意义.方法 采用胰胆管逆行穿刺注射牛磺胆酸钠建立不同炎症程度的急性胰腺炎模型,应用免疫组化、Western blot及RT-PCR等方法检测survivin基因在胰腺组织中的表达,TUNEL法检测胰腺泡细胞凋亡的变化.结果 正常胰腺组织无survivin的表达;建立急性胰腺炎模型后,随胰腺炎症程度的加重,Survivin蛋白和mRNA的表达水平逐渐升高,而腺泡细胞凋亡率则逐渐下降.结论 Survivin蛋白不仅通过其抗雕亡作用参与了急性胰腺炎时胰腺腺泡细胞凋亡的调节,而且通过其抗凋亡作用使受炎症损伤的胰腺细胞由凋亡转为坏死,从而加重急性胰腺炎病情,可能是预测急性胰腺炎严重程度的一个重要指标.
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| 关 键 词： | 凋亡 急性胰腺炎 |
Expression of survivin gene in a rat model of acute pancreatitis |
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| LI Zhen-dong,MA Qing-yong,LUO Yu-hong.Expression of survivin gene in a rat model of acute pancreatitis[J].Journal of Southern Medical University,2009,29(6):1141. |
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| Authors: | LI Zhen-dong MA Qing-yong LUO Yu-hong |
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| Institution: | LI Zhen-dong1,MA Qing-yong2,LUO Yu-hong1 1Department of Hepatobiliary Surgery,First Affiliated Hospital of Ji'nan University,Guangzhou 510630,China,2Department of Hepatobiliary Surgery,First Hospital of Xi'an Jiaotong University College of Medcine,Xi'an 710061 |
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| Abstract: | Objective To investigate the expression of survivin in acute pancreatitis in rats. Methods Acute pancreatitis was induced in rats by retrograde injection of sodium taurocholate into the pancreaticobiliary duct. The expressions of survivin in the pancreatic tissues was detected by immunohistochemisty, Western blotting and RT-PCR, and the apoptotic ratio of the acinar cells was determined by TUNEL assay. Results Survivin was not detected in the control group, and in rat models of acute pancreatitis, the expre... |
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| Keywords: | Survivin |
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