| Radiation and androgen withdrawal alter expression of apoptosis pathway genes of prostate cancer cells |
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| 作者姓名： | Hui-RongChen Shu-JieXia |
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| 作者单位： | [1]DepartmentofUrology,AffiliatedShanghaiFirstPeople＇sHospital,ShanghaiJiaotongUniversity,Shanghai200080,China [3]DepartmentofUrology,AffiliatedShanghaiFirstPeople＇sHospital,ShanghaiJiaotongUniversity,Shanghai200080,China |
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| 摘 要： | Aim: To investigate the altered expression of apoptosis pathway genes of prostate cancer cells treated by radiation and androgen withdrawal and whether the combined treatment may induce additive apoptosis. Methods: Androgen sensitive prostate cancer cell line LNCaP was cultured and treated by radiation, androgen withdrawal and combination of the two. Apoptosis was determined using apoptotic cells staining and mononuclear cell direct cytotox-icity assay. The total RNA were extracted and harvested. cDNA probes were prepared and labeled with biotin-16-dUTP and then hybridized to commercially available cDNA arrays, including apoptosis pathway-specific genes. The expression of important gene was further determined using RT-PCR. Results: Radiation induced additive apoptosis of prostate cancer cells; androgen withdrawal exhibited synergetic action. TNFRSF8 variant 2, DFFA, LTbR, mdm2, Myd88, TNFRSF14 and TNFSF4 mRNA were up regulated by radiation, while Survivin and Bar mRNA were down regulated. Mcl-1, TNFRSF14
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| 关 键 词： | 放射线 男性激素 合理服药 基因表达 细胞凋亡 遗传基因 前列腺癌 癌细胞 肿瘤 |
Radiation and androgen withdrawal alter expression of apoptosis pathway genes of prostate cancer cells |
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| Hui-RongChen Shu-JieXia.Radiation and androgen withdrawal alter expression of apoptosis pathway genes of prostate cancer cells[J].Asian Journal of Andrology,2004,6(3):258-258. |
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| Authors: | Hui-Rong Chen Shu-Jie Xia |
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| Institution: | Hui-Rong Chen,Shu-Jie XiaDepartment of Urology,Affiliated Shanghai First People'sHospital,Shanghai Jiaotong University,Shanghai 200080,China |
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| Abstract: | Aim: To investigate the altered expression of apoptosis pathway genes of prostate cancer cells treated by radiation and androgen withdrawal and whether the combined treatment may induce additive apoptosis. Methods: Androgen sensitive prostate cancer cell line LNCaP was cultured and treated by radiation, androgen withdrawal and combination of the two. Apoptosis was determined using apoptotic cells staining and mononuclear cell direct cytotox-icity assay. The total RNA were extracted and harvested. cDNA probes were prepared and labeled with biotin-16-dUTP and then hybridized to commercially available cDNA arrays, including apoptosis pathway-specific genes. The expression of important gene was further determined using RT-PCR. Results: Radiation induced additive apoptosis of prostate cancer cells; androgen withdrawal exhibited synergetic action. TNFRSF8 variant 2, DFFA, LTbR, mdm2, Myd88, TNFRSF14 and TNFSF4 mRNA were up regulated by radiation, while Survivin and Bar mRNA were down regulated. Mcl-1, TNFRSF14, MyD88 and TNFSF4 mRNA were up regulated by androgen withdrawal, while Bar, Survivin and TRAIL-R3 mRNA were down regulated. Conclusion: Radiation and androgen withdrawal altered the expression of apoptosis pathway genes of prostate cancer cells in different patterns, which may contribute to the additive apoptotic effect induced by the combined treatment. |
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| Keywords: | prostate cancer apoptosis signal pathway gene radiation |
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