多柔比星肾病大鼠podocin mRNA表达与氧化应激反应的关系

目的 探讨多柔比星肾病大鼠肾小球裂隙膜分子podocin mRNA表达与氧化应激反应的关系。方法 建立大鼠多柔比星肾病模型，原位杂交染色和半定量RT-PCR法检测肾皮质podocin mRNA表达，化学比色法检测肾皮质氧化应激指标，并对二者进行相关性分析。结果 原位杂交染色发现正常对照组podocin mRNA主要表达在肾小球细胞胞浆中。随时间进展多柔比星肾病组阳性细胞数和阳性强度明显增加；半定量RT-PCR结果显示肾病组podocin mRNA表达d7无明显改变，d14和d21显著升高。与正常对照组比较，多柔比星肾病组丙二醛（MDA）水平d7明显增高，d14和d21显著增高；超氧化物歧化酶（SOD）水平d14明显降低，持续至d21；总抗氧化能力（T-AOC）d21明显降低。podocin mRNA的表达与MDA呈正相关，与SOD和T-AOC呈显著负相关。结论 肾病状态下足细胞分子podocin mRNA表达异常与氧化应激反应密切相关。

Relationship between Expression of podocin mRNA and Oxidative Stress in Rats with Adriamycin-Induced Nephrotic Syndrome

Objective To explore the possible association of oxidative stress reaction with the mRNA expression of podocin in rats with adriarnycin(ADR)-induced nephrotic syndrome.Methods mRNA expression of podocin in renal cortex were investigated by in situ hybridization staining and semi-quantitative RT-PCR in ADR-induced nephrotic rats,the level of malondialdehyde(MDA), superoxide dismutase(SOD)and total antioxidative capacity(T-AOC) in renal cortex were measured, then the relationship between them was evaluated.Results In ADR rats, MDA increased at d7 and reached a significant higher level at d14 and d21;SOD decreased at d14 and persisted to d21; T-AOC decreased at d21.The podocin mRNA mostly expressed in cytoplasm of glomerular cells. Both the number and the intensity of positive cells increased notably as time progressed in ADR rats.The level of podocin mRNA expression showed no obvious changes at d7, while prominently increased at d14 and pesisted to d21 in ADR rats.There was significant correlativity between the mRNA expression of podocin and the level of MDA,SOD,T-AOC.Conclusion The mRNA expression of podocin is correlated with oxidative stress reaction in rats with ADR-induced nephrotic syndrome.