Effects of handling and etherization on incorporation of [3H] lysine into protein of mouse brain and liver |
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Authors: | Daniel J. Entingh Terri Damstra |
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Affiliation: | The Neurobiology Program, and The Division of Chemical Neurobiology Department of Biochemistry and Nutrition, School of Medicine University of North Carolina, Chapel Hill, NC 27514, USA |
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Abstract: | A standardized handling experience of 20 sec duration elevated the amount of radioactive lysine incorporated into brain total proteins during a 10 min labeling period begun either 10 min or 30 min after the handling. Etherization for 20 sec produced similar metabolic changes. Incorporation of [3H] lysine into liver proteins was minimally affected by handling, while slightly altered by etherization. The metabolic changes detected in the brain after handling did not appear to be side-effects of changes in blood-borne radioactivity. The results indicate that laboratory stresses often thought to be of minor importance can have large effects on ordinary assays of protein metabolism in brain. |
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Keywords: | Stimulation Protein Handling Etherization Brain Liver Lysine Radioactivity |
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