半枝莲的化学成分

目的对黄芩属植物半枝莲(Scutellaria barbataD.Don)的抗肿瘤活性部位(氯仿萃取物和正丁醇萃取物)的化学成分进行分离、鉴定。方法用体积分数为70%的乙醇加热回流提取干燥药材,经石油醚、氯仿、正丁醇分步萃取,利用反复硅胶柱色谱、聚酰胺柱色谱、Sephadex LH 20凝胶柱色谱进行分离、纯化,根据理化性质和核磁共振氢谱、碳谱数据对所得化合物进行结构鉴定。结果得到8个化合物,分别鉴定为对羟基苯甲醛(1)、3,4二羟基苯甲酸(2)、对羟基苯乙酮(3)、5,7,4′三羟基8甲氧基黄酮(4)、5,7,4′三羟基6甲氧基黄酮(5)、5,7,4′三羟基黄酮(6)、5,4′二羟基6,7,3′,5′四甲氧基黄酮(7)、5羟基4′甲氧基黄酮7OαL鼠李糖基(1→6)βD吡喃葡萄糖苷(8)。结论化合物7、8为半枝莲中首次分离得到。

Chemical constituents of Scutellaria barbata D.Don

Objective To study the active parts of the Scutellaria barbata D.Don and isolate and identify the chemical constituents from active parts.Methods The solvent extraction together with silica gel column chromatography were used for isolation of chemical constituents.Physicochemical characters and spectroscopic evidence were employed for the structural identification.Results Eight compounds were isolated,and identified as p-hydroxybenzaldehyde(1),3,4-dihydroxybenzoic acid(2),p-hydroxyacetophenone(3),5,7,4′-trihydroxy-8-methoxyflavone(4),5,7,4′-trihydroxy-6-methoxyflavone(5),5,7,4′-trihydroxyflavone(6),5,4′-dihydroxy-6,7,3′,5′-tetramethoxyflavone(7),5-hydroxy-4′-methoxyflavone-7-O-α-L-rhamnosyl-(1→6)-β-D-glucopyranoside(8),respectively.Conclusion Compound 7,8 are isolated from Scutellaria barbata D.Don for the first time.