重组人脑钠肽对兔缺血再灌注后心室肌细胞L-钙离子通道电流的影响 |
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引用本文: | 丁超,郭洁,王冬梅,李洁,陈会校,孙家安.重组人脑钠肽对兔缺血再灌注后心室肌细胞L-钙离子通道电流的影响[J].中国循证心血管医学杂志,2013,5(1):73-75. |
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作者姓名: | 丁超 郭洁 王冬梅 李洁 陈会校 孙家安 |
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作者单位: | 解放军白求恩国际和平医院心内科 |
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摘 要: | 目的研究重组人脑钠肽(rhBNP)对缺血再灌注后心室肌细胞L-钙通道电流(ICa-L)的影响,并探讨其细胞学离子机制。方法 45只新西兰大耳白兔随机分为缺血再灌注动物模型组(I-R组,n=15)、rhBNP治疗组(n=15)和假手术组(n=15)。采用酶解方法分离缺血部位心室肌外膜单个心室肌细胞,应用全细胞膜片钳技术记录ICa-L。结果①心律失常发生率:与I-R组比较,rhBNP组兔室速、室颤发生率及持续时间明显下降,而且其心律失常的评分也明显低于I-R组(2.6±0.7)vs.(3.6±0.8),P〈0.05];②电流密度峰值:I-R组、对照组、rhBNP组ICa-L电流密度峰值(0mV)逐渐升高,分别为(-4.34±0.92)pA/pF、(-3.42±0.76)pA/pF、(-3.13±1.22)pA/pF。结论 rhBNP可降低心肌缺血再灌注期间心律失常的发生率,心肌缺血再灌注后ICa-L明显增高,rhBNP可使ICa-L下调,逆转电重构。
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关 键 词: | 重组人脑钠肽 缺血 再灌注 膜片钳 离子通道 兔 |
Influence of recombinant human brain natriuretic peptide on current of ventricular myocyte L-type calcium channels in rabbits after myocardial ischemia-reperfusion |
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Authors: | DING Chao GUO Jie WANG Dongmei LI Jie CHEN Hui-xiao SUN Jia-an |
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Institution: | .* Department of Cardiology,Bethune International Peace Hospital,Shijiazhuang 050082,China. |
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Abstract: | Objective To establish rabbit model of myocardial ischemia/reperfusion,study the influence of recombinant human brain natriuretic peptide(rhBNP) on current of ventricular myocyte L-type calcium channels(I Ca-L),and explore the ionic mechanism.Methods New Zealand rabbits(n=45)were randomly divided into model group(n =15),treatment group(n=15)and sham-operation group(n=16).Single ventricular myocytes were isolated by using enzymolysis method from ventricular epicardium in ischemia area.The current of I Ca-L was recorded by using whole-cell patch clamp technique.Results ① Incidence of arrhythmia:the incidence and duration of ventricular tachycardia and ventricular fibrillation,and scores of arrhythmia all decreased significantly in treatment group compared with model group (2.6 ± 0.7)vs.(3.6 ± 0.8),P〈0.05].② The peak value of I Ca-L current density(0 mV)increased gradually in model group,control group and treatment groups (-4.34 ± 0.92)pA/pF,(-3.42 ± 0.76)pA/pF and(-3.13 ± 1.22)pA/pF].Conclusion During the stage of myocardial ischemia/reperfusion,rhBNP can reduce the incidence of arrhythmia.The current of I Ca-L increases significantly after myocardial ischemia/reperfusion,while rhBNP can down-regulate increased I Ca-L current and reverse electrical remodeling. |
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Keywords: | Recombinant human brain natriuretic peptide Ischemia/reperfusion Patch clamp Ionic channel Rabbits |
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