山楂叶总黄酮对大鼠心肌缺血再灌注损伤的心电图、一氧化氮及丙二醛变化的影响

目的：观察山楂叶总黄酮（hawthorn leaves flavonoids，HLF）对大鼠心肌缺血再灌注损伤的心电图、NO及丙二醛（MDA）变化的影响。方法：结扎大鼠冠状动脉左前降支，使心肌缺血30min，再灌注60min，制备急性心肌缺血再灌注损伤模型，缺血前10min，腹腔注射HLF（12．5、25．0、50．0mg／kg）。动态观察心电图，同时监测心电图ST段的变化，用硝酸还原酶法测定血清中NO浓度，采用硫代巴比妥钠（TAB）比色法测定组织内MDA含量。结果：HLF（12．5、25．0、50．0mg／kg）能减轻缺血再灌注损伤心肌心电图ST段变化，提高血清NO的浓度，降低心肌缺血再灌注时心肌组织MDA含量。结论：HLF能改善心肌缺血再灌注损伤心电图变化，对损伤心肌有保护作用，其机制与增加NO浓度、抗脂质过氧化的作用有关。

Influence of hawthorn leaves flavonoids on electrocardiogram, nitrogen monoxidum and malondia1dehyde of myocardial ischemia reperfusion injury in rats

AIM:To study the influence of hawthorn leaves flavonoids on electrocardiogram (ECG), NO and malondialdehyde (MDA) of myocardial ischemia reperfusion injury in rats. METHODS: Myocardial ischemic reperfusion injury models were established by occluding the left anterior descending branch of coronary artery for 30 min and removing the ligation later to reperfuse for 60 min in vivo. Different dosages of hawthorn leaves flavonoids(12.5, 25.0, 50.0 mg/kg) were administered by i.p. 10 min before the onset of ischemia. ECG was observed continuously and ST segments were measured. The concentrations of NO in blood serum were detected and the contents of MDA in myocardium were assaied in the same time. RESULTS: ST segments of ECG were relieved significantly treated with hawthorn leaves flavonoids (12.5, 25.0, 50.0 mg/kg). NO concentrations were increased and MDA contents were decreased significantly in the different dosages groups compared with control group. CONCLUSION: Hawthorn leaves flavonoids has protective effects on myocardial ischemia reperfusion injury.The effect were possibly attributed to increasing concentrations of NO and anti-lipid peroxidation.