远隔肢体缺血后适应对大鼠 MCAO 模型血管重塑的影响

目的：研究缺血后适应对大鼠急性缺血性脑卒中脑血流恢复、脑血管直径及脑梗死体积的影响。方法将SD大鼠随机分为后适应组（ MCAO＋后适应）、对照组（ MCAO）及假手术组（ Sham）。线栓法建立大鼠永久性MCAO缺血模型，于缺血前、缺血第1天及缺血第7天对对照组和后适应组进行脑血流检测，于缺血第1天和第7天行神经功能评分，在缺血第7天行脑梗死体积检测、大脑前动脉和大脑后动脉直径检测。结果缺血第1天后适应组与对照组神经功能评分无明显差异（P＞0．05），第7天实验组较对照组神经功能明显改善（P＜0．05）；第7天后适应组较对照组脑梗死体积明显缩小（P＜0．01）；第7天对照组侧支血管直径较Sham组明显增粗（P＜0．01），后适应组侧支血管直径较Sham组明显增粗（P＜0．01），后适应组血管直径与对照组相比明显增粗（P＜0．05）；缺血第1天和第7天实验组脑血流量较对照组均有明显提高（P＜0．05）。结论实验结果显示缺血后适应能提高神经功能评分，减少脑梗死体积，促进缺血早期脑血流恢复，促进脑血管重塑。

The Protective of Limb Remote Ischemic Postconditioning on Capillary Remodeling after Permanent MCAO in Mice

Objective To study the effect of Limb Remote Ischemic Postconditioning on cerebral blood flow restoration ,diame-ters of collateral arteries and cerebral infarction after permanent MCAO in mice .Methods SD rats were randomly divided into postcondition-ing group,ischemia control group and sham group .Suture method was used in MCAO .Cerebral blood flow was measured repeatedly at day 1 （ before and immediately after ischemia ） and day 7 at the same region .Neurological functional deficits were evaluated at day 1 after ischemia and day 7.The diameter of anterior cerebral arteries and posterior cerebral arteries were measured by a stereo -zoom microscope.And the cere-bral infarction volumes were measured at day 7.Results After 7 days,compared with the control group ,the functional neurological outcome of ischemia postconditioning group was improved significantly （P〈0.05）,while on day 1 the functional neurological outcome of ischemia con-trol group and ischemia postconditioning group had no significant difference （ P〉0.05）.After 7 days,compared with the ischemia control group,the infarction volumes of postconditioning group significantly reduced （P〈0.01）.After 7 days,the diameters of the collateral arteries were significantly larger than the non-ischemia control group（P〈0.01）,and the diameters of the ischemia postconditioning group were larger than sham group（P〈0.01）.The diameters of the ischemia postconditioning group were larger than ischemia group （P〈0.05）.On day 1 and day 7,cerebral blood flow of the ischemia postconditioning group was significantly improved compare with the ischemia control group （ P〈0. 05 ） .Conclusion Ischemia postconditioning can improve the functional neurological outcome and reduce the cerebral infarction volumes ,and it also can restore the cerebral blood flow earlier and mediate vascular remodeling .