Use of a new oligonucleotide probe for detection of colonization factor antigen III gene in enterotoxigenicEscherichia coli |
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| Authors: | T Taniguchi N F Uchima-Senaga Y Takarada S Shibata T Tsukamoto K Yamamoto T Honda |
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| Institution: | (1) Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita, 565 Osaka, Japan;(2) Toyobo Research Center, Katada, Ohtsu, 520-02 Shiga, Japan;(3) Osaka Public Health Laboratory, Higashiku, 537 Osaka, Japan |
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| Abstract: | An alkaline phosphatase-labeled 30-mer oligonucleotide probe was designed to detect the gene for pilus colonization factor antigen III (CFA/III) of the human type of enterotoxigenicEscherichia coli (ETEC). The CFA/III probe was used to identify CFA/III-producing ETEC among 303Escherichia coli obtained from subjects with traveler's diarrhea. Six isolates positive for the CFA/III gene were found. This result was confirmed immunologically by using a specific monoclonal antibody developed against CFA/III. These six isolates, isolated from travelers returning from India, Pakistan and China, were all positive for the gene of heat-labile enterotoxin and possessed an identical serotype (025:H–). |
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