| ELISA法检测人抗鼠抗体的研究 |
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| 引用本文： | 王娟,史敏,李晓军,武建国. ELISA法检测人抗鼠抗体的研究[J]. 医学研究生学报, 2001, 14(Z1): 29-31 |
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| 作者姓名： | 王娟 史敏 李晓军 武建国 |
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| 作者单位： | 南京军区南京总医院解放军医学检验中心,江苏南京,210002 |
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| 摘 要： | 目的对1000例健康人和284例全身性红斑狼疮(SLE)、类风湿性关节炎(RA)、肾病、肿瘤患者中人抗鼠抗体(HAMA)进行普筛，并对其产生情况进行初步探讨。方法用酶联免疫吸附(ELISA)法检测待测血清中的HAMA。结果1000例正常健康人HAMA阳性检出率为1.1%，76例SLE、40例RA、60例肾病、108例肿瘤疾病中HAMA阳性检出率分别为7.9%、5.0%、8.3%和7.4%。结论肿瘤患者的HAMA阳性率与正常对照组相比差异显著(P<0.01)，这可能是肿瘤患者较其他疾病组有较多机会接触鼠源性单抗治疗有关，其他疾患组总体阳性值未发现有统计学意义。
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| 关 键 词： | 人抗鼠抗体；酶联免疫吸附法；全身性红斑狼疮；类风湿性关节炎；肾病；肿瘤 |
| 文章编号： | 1008-8199(2001)增刊-0029-02 |
| 修稿时间： | 2000-11-03 |
Screening the human antimouse antibodies with ELISA |
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| WANG Juan,SHI Min,LI Xiao-jun,WU Jian-guo. Screening the human antimouse antibodies with ELISA[J]. Bulletin of Medical Postgraduate, 2001, 14(Z1): 29-31 |
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| Authors: | WANG Juan SHI Min LI Xiao-jun WU Jian-guo |
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| Abstract: | Objectives:To screen human anti mouse antibodies(HAMA) in sera from 284 patients with SLE, RA, tumors and nephropathy as well as 1000 healthy subjects and explore the conditions of their production. Methods:ELISA was used to detect HAMA in serum. Results:The positive rates of HAMA were 7.9%, 5.0 %,8.3% and 7.4% in sera from SLE, RA, tumors and nephropathy respectively while 1.1% in healthy subjects. Conclusions:A significant difference ( P <0.01) was found between tumor patients, those receiving mouse source monoclonal antibodies and other patient groups. There were no statistical differences of positive rates among other groups. |
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| Keywords: | Human anti mouse antibody ELISA SLE RA Nephropathy Tumor |
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