生理盐水与人血清做稀释剂对钒酸盐氧化法测定直接胆红素的影响

目的探讨生理盐水与人血清做稀释介质对钒酸盐法测定直接胆红素的结果影响。方法待测血清分别用生理盐水和人血清稀释后用钒酸盐氧化法测定直接胆红素。结果待测血清经人血清或生理盐水稀释后测定直接胆红素，结果差异有统计学意义（P〈0．01），两种稀释方法测定结果差异有统计学意义（P〈0．01），用血清稀释测定结果偏差低于生理盐水稀释法。结论钒酸盐氧化法测定直接胆红素，用生理盐水稀释样本后测定结果明显增高，对超检测线范围的离浓度直接胆红素样本用血清介质稀释优于用生理盐水稀释测定。

Influence of physiological saline with human serum as diluent on direct bilirubin detection with vanadate oxidation method

Objective To investigate the influence of physiological saline and human blood serum as the dilution medium on direct bilirubin detection with vanadate oxidation method.Methods The concentration of direct bilirubin in serum was determined with the vanadate oxidation method after the serum samples were diluted with physiological saline and the human serum respectively.Results The concentration of direct bilirubin had significant difference between human serum and physiological saline diluted(P<0.01).There was significant difference bilirubin concentrations between the two diluent methods(P<0.01).The deviation of serum diluent was smaller than that of physiological saline diluent.Conclusion The direct bilirubin concentration is significantly increasing when the sample diluted by physiological saline in vanadate oxidation method,and human serum diluent is better than physiological saline in highly concentrated direct bilirubin samples surpassing the measuring linear limit.