结核/非结核分枝杆菌核酸快速检测方法临床应用价值

目的 评价结核/非结核分枝杆菌核酸快速检测方法的临床应用价值。 方法 应用实时荧光PCR技术对420例标本进行临床试验研究，并与抗酸杆菌涂片和分枝杆菌培养方法对照比较。 结果 结核分枝杆菌核酸检测灵敏度为48.5%（其中菌阳标本灵敏度为95.8%，菌阴标本灵敏度为14.5%），特异度99.3%，阳性预测值为99.1%，阴性预测值为55.5%；临床试验388例痰标本中检测出1例非结核分枝杆菌核酸阳性，经测序确认，准确率100%；同时对32例非结核分枝杆菌临床分离株进行核酸检测，并经测序确认，准确率100%。 结论 应用实时荧光PCR技术，能实现在1支管内同时进行结核分枝杆菌/非结核分枝杆菌的核酸检测。该方法具有简单快速、特异性好污染性少的特点。可作为实验室辅助检测结核/非结核分枝杆菌核酸方法之一。

Clinical value of real-time FQ-PCR assay for detection of Mycobacterium tuberculosis/non-tuberculous mycobacteria

Objective To evaluate the clinical diagnostic value of real-time FQ-PCR assay for the detection of Mycobacterium tuberculosis(MTB)/non-tuberculous mycobacteria(NTM). Methods 420 specimens were detected by real-time FQ-PCR,and the results were compared with those of AFB smear and culture. Results The sensitivity of FQ-PCR was 48.5%,in which the sensitivity in bacterium-positive and bacterium-negative specimens were 95.8% and 14.5%,respectively.The specificity,positive predictive value and negative predictive...