端粒酶催化亚单位和c-Myc蛋白在中耳胆脂瘤上皮中的表达及意义

目的:观察端粒酶催化亚单位(TERT)和癌基因cMyc的表达产物(cMyc蛋白)在中耳胆脂瘤上皮中的表达情况,分析其在胆脂瘤上皮增殖演变过程中的作用。方法:应用SP法和计算机图像分析系统,检测TERT、cMyc蛋白在32例胆脂瘤组织和14例胆脂瘤患者外耳道皮肤中的表达情况,并进行比较。结果:TERT和cMyc蛋白在所有胆脂瘤上皮各层细胞均存在较强表达,外耳道皮肤表达较弱。各组间胆脂瘤上皮各指标平均阳性细胞率和平均吸收度(平均灰度的倒数),均高于外耳道皮肤,差异有统计学意义(P<0.05);皮下结缔组织伴有大量的炎症细胞,如:淋巴细胞、浆细胞、中性粒细胞和巨噬细胞;胆脂瘤上皮中TERT和cMyc蛋白之间存在正相关关系(P<0.01)。结论:TERT和cMyc蛋白在胆脂瘤上皮的表达从不同方面反映了胆脂瘤上皮的过度增生和角化,二者在胆脂瘤的发病过程中有协同作用;上皮下炎症反应可能是角化细胞过度增殖的一个原因。

Expression and significance of TERT and c-Myc oncogene protein product on cholesteatoma epithelium

Objective:To detect the expression and localization of human telomerase catalytic subunit(hTERT) and c-Myc oncogene protein product in human middle ear cholesteatoma,and to evaluate their roles in the proliferation of cholesteatoma epithelium.Method:The specimens from the middle ear cholesteatoma tissue of 32 cases and external ear skin of 14 cases were examined by immunohistochemical SP method and computer image analysis system for TERT and c-Myc oncogene protein product.Result:All 32 samples of cholesteatoma showed a stronger expression of TERT and c-Myc oncogene protein product than that of the external ear skin.The indexes,such as mean positive cell rate and mean obsorbency,of cholesteatoma epithelium are much higher than that of the normal external ear shin remarkably(P<0.05).Accompanied with numerous inflammatory cells(lymphocytes,plasmocytes,neutrophils and macrophages) subcutaneously, the high expression of TERT and c-Myc oncogene protein product were correlated with each other(P<0.05).Conclusion: Excessive proliferation and keratinization of cholesteatoma are presented by the expression of TERT and c-Myc oncogene protein product in different ways,both of which play a cooperative role in the development of cholesteatoma.Subcutancous inflammatony response may contribute to the excessive proliferation of keratose cells.