羟基磷灰石纳米粒子对肝癌H22小鼠肿瘤细胞凋亡的研究

目的通过不同浓度的羟基磷灰石(HAP)纳米粒子作用于肝癌H22小鼠肿瘤细胞,研究其对肿瘤细胞凋亡的影响。方法100只肝癌H22小鼠随即分组并连续服用不同剂量的HAP纳米粒子,11d后处死小鼠,计算抑瘤率和细胞凋亡率,评价不同剂量的HAP纳米粒子与H22小鼠肿瘤细胞生长的关系。结果肝癌H22小鼠肿瘤细胞对纳米羟基磷灰石(HAP)较敏感,能明显延长小鼠生存期,高剂量组与丝裂霉素结果相近(P>0.05)。实验后小鼠体重没有明显下降,抑瘤率和细胞的凋亡率随浓度升高而增大,呈明显的剂量依赖性(P<0.01)。结论不同浓度的羟基磷灰石(HAP)纳米粒子能够抑制H22肿瘤细胞的增殖,诱导肿瘤细胞的凋亡,并呈剂量依赖性。

Effects of hydroxyapatite ceramic nano-particles on apoptosis of H22 tumor cell line of mice hepatic cancer

Objective To explore the effects of different dosage of hydroxyapatite ceramic(HAP) Nano-particles on apoptosis of H22 tumor cell line in mice hepatic cancer.Methods 100 mice with tumor of H22 were divided into groups randomly,which taken different dosage of HPA Nano-particles in different group one time a day.After 11 days,all the mice were sacrificed and the inhibition rate and the cell apoptosis were calculated to evaluate the relationship between dosage of HAP Nano-particles and tumor growth.Results Tumor of H22 were sensitive to HAP Nano-particles,and could also prolong the life span of mice(P<0.01).The results was approximate to that of mitomycin group(P>0.05)。The weight of the mice did not decrease.The rate of apoptosis and that of growth inhibiting increased with the rising concentration of the HAP Nano-particles.Conclusion HAP Nano-particles can induce apoptosis of the tumor H22 cell line in mice,which is dose-dependent.