| HPLC法测定注射用水溶性维生素中维生素B_(12)和生物素的含量 |
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| 引用本文： | 曹玮,刘梅妍,冉俐. HPLC法测定注射用水溶性维生素中维生素B_(12)和生物素的含量[J]. 中国药房, 2007, 18(7): 543-545 |
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| 作者姓名： | 曹玮 刘梅妍 冉俐 |
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| 作者单位： | 1. 云南省人民医院药剂科,昆明市,650032
2. 四川大学华西药学院,成都市,610041 |
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| 摘 要： | 目的:建立以高效液相色谱法同时测定注射用水溶性维生素中维生素B12和生物素含量的方法。方法:色谱柱为Phe-nomenexC18,检测波长为210nm,流动相为0.1%磷酸-甲醇(81∶19),流速为1.0mL·min-1,柱温为35℃。结果:维生素B12和生物素线性范围分别为0.0035～0.0245μg(r=0.9997)和0.03476～0.24332μg(r=0.9992),平均回收率分别为99.75%、99.91%(RSD=1.40%、1.95%)。结论:本方法较简便、快速,准确度较高,可用于控制该制剂的质量。
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| 关 键 词： | 高效液相色谱法 水溶性维生素 维生素B12 生物素 含量测定 |
| 文章编号： | 1001-0408(2007)07-0543-03 |
| 修稿时间： | 2006-10-11 |
Determination of Vitamin B12 and Biotin in Water- soluble Vitamin for Injection by HPLC |
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| CAO Wei,LIU Meiyan,RAN Li. Determination of Vitamin B12 and Biotin in Water- soluble Vitamin for Injection by HPLC[J]. China Pharmacy, 2007, 18(7): 543-545 |
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| Authors: | CAO Wei LIU Meiyan RAN Li |
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| Abstract: | OBJECTIVE: To establish an HPLC method to determine the content of vitamin B12 and biotin in water-soluble vitamins for injection. METHODS: Phenomenex C18 was used as the column, with the detection wavelength set at 210nm, the mobile phase was 0.1% phosphoric acid-methanol (81∶19), with the flow rate at 1.0mL·min-1 and the oven temperature at 35℃. RESULTS: Vitamins B12 and biotin linear range was 0.003 5～0.024 5μg(r=0.999 7) and 0.0347 6～ 0.243 32μg(r=0.999 2) respectively. The average recovery was 99.75% and 99.91% respectively(RSD=1.40% and 1.95%). CONCLUSION: The method was simple, rapid and accurate. |
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| Keywords: | HPLC Water-soluble vitamin Vitamin B12 Biotin Content determination |
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