卵巢浆液性肿瘤中凋亡调控因子Smac Caspase-9和Caspase-3的表达及意义

目的：探讨凋亡调控因子Smac、Caspase-9和Caspase-3在卵巢浆液性肿瘤中的表达。方法：采用免疫组织化学技术（SP法）和图像分析技术检测25例卵巢浆液性囊腺癌、10例卵巢交界性浆液性囊腺瘤、30例卵巢浆液性囊腺瘤和24例正常卵巢组织中凋亡调控因子Smac、Caspase-9和Caspase-3表达水平。结果：Caspase-3、Caspase-9和Smac在30例浆液性囊腺瘤中的表达率分别为93%、93%和73.3%;在10例交界性浆液性囊腺瘤中的表达率分别为70%、80%和80%;在25例浆液性囊腺癌中的表达率分别为96%、96%和84%。阳性信号主要定位于细胞质,呈弥漫分布。而在正常卵巢24例中的表达率分别为83.3%、80.9%和58.3%。凋亡调控因子Caspase-3、Caspase-9和Smac在浆液性卵巢肿瘤中的表达增加（P〈0.05）。结论：凋亡调控因子Caspase-3、Caspase-9和Smac在卵巢浆液性肿瘤中的表达增加。这提示Caspase-3、Caspase-9和Smac可能会成为卵巢浆液性肿瘤治疗的新靶点。

Expression of Apoptosis-regulatory Factors Caspase-3, Caspase-9 and Smac in Serous Ovarian Neoplasms

Objective: To investigate the action of caspase-3, caspase-9 and smac, regulatory factors of apoptosis, in serous ovarian neoplasms. Method: Immunohistochemical staining (S-P) and image analysis were used to assess the expression levels of caspase-3, caspase-9 and smac in 30 cases of serous cystadenoma, 10 cases of borderline serous cystadenoma, 25 cases of serous cystadenocarcinoma and 24 specimens from normal ovary. Results: The expression of caspase-3, caspase-9 and smac was 93%, 93% and 73.3%, respectively, in the 30 cases of serous ovarian cystadenoma; 70%, 80% and 80%, respectively, in the 10 cases of borderline serous cystadenoma; 96%, 96% and 84%, respectively, in the 25 cases of serous cystadenocarcinoma; and 83.3%, 80.9%, and 58.3%, respectively, in the 24 normal ovary specimens. Expression levels of caspase-3, caspase-9 and smac in serous ovarian neoplasms were higher than those found in normal ovary (P<0.05). Conclusion: Expression levels of apoptosis-regulatory factors caspase-3, caspase-9 and smac are increased in serous ovarian neoplasms, indicating that these genes may be new targets for tumor treatment.