| 环孢菌素A逆转白血病多药耐药机制的实验研究 |
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| 引用本文： | 赵春亭,杨纯正,杨天楹,邵晓枫,熊冬升,许元富,齐静.环孢菌素A逆转白血病多药耐药机制的实验研究[J].白血病．淋巴瘤,2001,10(2):68-70. |
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| 作者姓名： | 赵春亭 杨纯正 杨天楹 邵晓枫 熊冬升 许元富 齐静 |
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| 作者单位： | 1. 山东青岛大学医学院附属医院
2. 中国医学科学院中国协和医科大学血液学研究所,天津,300020 |
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| 摘 要： | 目的：研究环孢菌素A（CsA)持续作用7天后，K562／AO2细胞多药耐药性的变化及其变化机制，方法：细胞毒性试验采用MTTI地，细胞内柔红霉素（DNR）浓度用流式细胞术检测，多药耐药基因（mdrl)的表达水平用RT－PCR方法检测，结果：K562／AO2耐药性降低2．2倍，K562／AO2细胞内DNR浓度明显升高，mdrl表达降低，佛伯酯（TPA）可拮抗CsA升高细胞的DNR浓度的作用，结论：CsA可下调mdrl的表达和细胞内DNR浓度进而逆转MDR，其作用可能与CsA抑制蛋白激酶D（PKC）活性有关。
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| 关 键 词： | 多药耐药 白血病 环孢菌素A 治疗 |
| 文章编号： | 1009-9921(2001)02-0068-03 |
| 修稿时间： | 2000年7月22日 |
EXPERIMENTAL STUDY ON REVERSAL MECHANISM OF MULTIDRUG RESISTANCE BY CYCLOSPORINE A |
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| ZHAO Chun-ting,YANG Chun-zheng,YANG Tian-ying,et al Institute of Hematology,CAMS and PUMC.EXPERIMENTAL STUDY ON REVERSAL MECHANISM OF MULTIDRUG RESISTANCE BY CYCLOSPORINE A[J].Journal of Leukemia & Lymphoma,2001,10(2):68-70. |
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| Authors: | ZHAO Chun-ting YANG Chun-zheng YANG Tian-ying Institute of Hematology CAMS and PUMC |
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| Abstract: | Objective:To investigate the variation of multidrug resistance (MDR) in K562/AO2 cells after the sustaining affection on K562/AO2 cells by cyclosporine A.Methods:Cytotoxic effect was assayed by MTT method after K562/AO2 cells were cultured in CsA-containing medium for 7 days.Flow cytometry was used to mersured the intracellular daunorubicin concentration.The expression of MDR gene was analysied by RT-PCR method.Rusults:The sensitivity of to K562/AO2 cells to DNR increased by about 2 times with the increasing of intracellular DNR concentration which can be antagonized by phorbol ester (TPA) and the decreasing of mdr1 gene expression.Conclusion:CsA could reverse the MDR in K562/AO2 cells by down-regulation the expression of mdr1 gene and increasing the intracellular drug concentration.These might be contributed to it's inhibiting effect on protein kinase C(PKC) activity. |
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| Keywords: | Multidrug resistance Leukemia Cyclosporine A |
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