| 反相高效液相色谱法测定盐酸帕洛诺司琼注射液的含量 |
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| 引用本文： | 方滢芝,杨进,郑国钢. 反相高效液相色谱法测定盐酸帕洛诺司琼注射液的含量[J]. 中国药学杂志, 2007, 42(6): 473-474 |
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| 作者姓名： | 方滢芝 杨进 郑国钢 |
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| 作者单位： | 1. 浙江大学医学院附属邵逸夫医院,杭州,310016
2. 浙江省药品检验所,杭州,310004 |
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| 摘 要： | 目的建立盐酸帕洛诺司琼注射液含量测定的高效液相色谱方法。方法采用Alltima C18色谱柱(4.6 mm×250 mm,5μm),以0.02 mol·L-1磷酸二氢钠溶液(含0.25%的三乙胺与0.01 mol·L-1己烷磺酸钠,用磷酸调节pH值至3.0)-乙腈(40∶60)为流动相;检测波长为254 nm,流速为1.5 mL·min-1。结果线性范围为10.3～103.1 mg·L-1,r=0.999 9;平均回收率为100.4%,RSD为0.95%(n=9)。结论本方法简便、可靠,可用于盐酸帕洛诺司琼注射液的含量测定。
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| 关 键 词： | 高效液相色谱法 盐酸帕洛诺司琼注射液 含量测定 |
| 文章编号： | 1001-2494（2007）06-0473-03 |
| 收稿时间： | 2006-01-20; |
| 修稿时间： | 2006-01-20 |
Determination of Palonosetron Hydrochloride for Injection by HPLC |
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| FANG Ying-zhi,YANG Jin,ZHENG Guo-gang. Determination of Palonosetron Hydrochloride for Injection by HPLC[J]. Chinese Pharmaceutical Journal, 2007, 42(6): 473-474 |
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| Authors: | FANG Ying-zhi YANG Jin ZHENG Guo-gang |
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| Affiliation: | 1. Affiliated Sir Run Run Shaw Hospital, Medicine College, Zhejiang University, Hangzhou 310016 ,China;2. Zhejiang Provincial Institute for Drug Control , Hangzhou 310004 ,China |
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| Abstract: | OBJECTIVE To establish a HPLC method for the determination of palonosetron hydrochloride for injection.METHODS The separation was performed on a Alltima C18 column(4.6 mm×250 mm,5 μm)with the mobile phase consisting of 0.02 mol· L-1 sodium dihydrogen phosphate(contain 0.25% triethylamine and 0.01 mol·L-1 sodium hexanesulfonate,adjust the pH to 3.0 with phosphoric acid)-acetontrile(40∶60).The detection wavelength was 254 nm.RESULTS The method was proved to be linear in the range of 10.3～103.1 mg·L-1,the regression coefficient was 0.999 9.The recovery of method was 100.4%(RSD 0.95%,n=9).CONCLUSION The method is simple,reliable and suitable for the determination of palonosetron hydrochloride for injection. |
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| Keywords: | HPLC palonosetron hydrochloride for injection assay |
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