均匀设计法优化塔斯品碱脂质体处方

 目的优化塔斯品碱脂质体的制备处方。方法采用薄膜挤压法制备塔斯品碱脂质体,根据均匀实验设计,以外观、脂质体粒径、复溶情况及包封率为指标综合评价。结果塔斯品碱脂质体的最佳制备处方为:蛋黄卵磷脂与胆固醇摩尔比50:50;水合介质用量10 mL;冻干保护剂选用蔗糖,用量为13%。所得塔斯品碱脂质体的平均粒径为186.4 nm,包封率为(88.29±4.2)%。结论由最佳处方制备的塔斯品碱脂质体,粒径均匀,包封率较高。

Application of Uniform Design for Preparation of Taspine Liposomes

OBJECTIVE To optimize the preparation formulation of the taspine liposomes.METHODS The taspine liposomes were prepared by the film evaporation-extrusion.The technology was optimized by unilorm design,and evaluated by taspine liposomal appearance,partical size distribution,redissolution and entrapment efficiency.RESULTS The results demonstrated that the best for- mulation of the taspine liposomes was as tollows:the mol ratio of egg phosphatidylcholine to cholesterol was 50:50,the volume of the hydration solution was 10 mL and the concentration of sucrose as a lyoprotectant was 13%.The mean size of liposomes determined by the particle size analyzer was 186.4 nm and the entrapment efficiency was(88.29±4.2)%.CONCLUSION The taspine liposome prepared according to the hest formulation has good morphological characteristics and high entrapment efficiency.