异丙酚对缺血再灌注损伤神经细胞突触素表达的影响

目的 研究异丙酚对缺血再灌注损伤神经细胞的保护及海马原代细胞和脑组织中突触素的表达情况.方法 取12h新生Wistar大鼠海马细胞体外培养,建立原代海马细胞缺糖缺氧再给氧模型(oxygen glucose deprivation,OGD).流式细胞仪检测应用异丙酚对海马细胞OGD后死亡和凋亡的影响,免疫细胞化学观察海马细胞中突触素(synaptophysin)的表达.建立SD大鼠脑缺血再灌注损伤的大脑中动脉阻断(middle cerebral artery occlusion,MCAO)模型,分为模型组、异丙酚用药组、正常对照组.观测各组动物术后7d、14d的体重变化及神经症状评分;将术后14d大鼠取脑,观测皮质中突触素的表达.结果 海马细胞缺糖缺氧再给氧显示异丙酚用药组海马细胞存活数量较多、凋亡细胞减少,海马细胞中突触素表达显著增强.大鼠模型7d体重变化异丙酚用药组为(285.0±1.6)g较模型组(165.0±8.6)g显著降低(P<0.01).14d体重变化异丙酚用药组为(304.7±8.6)g较模型组(182.5±23.5)g显著降低(P<0.01).7d神经症状评分异丙酚用药组为1.17±0.23,较模型组1.83±0.24显著降低(P<0.05).14d评分异丙酚用药组为1.00±0.41较模型组2.33±0.47也显著降低(P<0.05).免疫染色可见异丙酚用药组大脑皮质突触素的表达相较模型组明显增强.结论 细胞和动物模型均显示异丙酚对缺血再灌注损伤后神经细胞具有保护作用,并可增加皮质神经细胞中突触素的表达.

Effect of propofol on expression of synaptophysin in nerve cells after ischemia reperfusion injury

Objective To study the effect of propofol on nerve cells after ischemia reperfusion(I/R) injury and expression of synaptophysin in hippocampal primary cells and cerebral tissue.Methods A model of rat oxygen-and glucose-deprived(OGD) and re-oxygenated hippocampal primary cells was established using in vitro cultured hippocampal cells from 12h-old Wistar rats.Effect of propofol on death and apoptosis of hippocampal cells was detected by flow cytometry and expression of synaptophysin in hippocampal cells was observed with immunocytochemical staining.A rat middle cerebral artery occlusion(MCAO) model was established after I/R injury.The animals were divided into MCAO model group,propofol treatment group,and normal control group.Their body weight and neurological symptom score were observed on day 7 and 14 after operation.Expression of synaptophysin in cortex of rats was observed on day 14 after operation.Results The number of surviving OGD and re-oxygenated hippocampal primary cells was greater,the number of apoptotic cells was smaller,and the expression level of synaptophysin was higher after propofol treatment than before propofol treatment(P<0.01).The body weight was significantly higher in propofol treatment group than in MCAO group 7 and 14 days after operation((285.0±1.6) vs(165.0±8.6)g,(304.7±8.6) vs(182.5±23.5)g,P<0.01). The neurological symptom score was lower in propofol treatment group than in MCAO model group 7 and 14 days after operation((1.17±0.23) vs(1.83±0.24),(1.00±0.41) vs(2.33±0.47),P<0.05).Immunohistochemical staining showed that the expression level of synaptophysin was significantly higher in propofol treatment group than in MCAO model group(P<0.05).Conclusion Propofol can protect nerve cells after cerebral I/R injury and increase the expression of synaptophysin in nerve cells of cotex.