高温高湿环境对大鼠创伤性脑水肿的影响

目的 通过研究实验大鼠颅脑损伤后创伤区脑含水量变化情况及脑组织水通道蛋白4表达的影响,分析高温高湿环境对创伤性脑水肿的影响.方法 选择成年健康雄性SD大鼠64只,随机分为常温无创组16只(25℃、RH 50％),常温创伤组(16只),高温高湿无创伤组16只(35℃、RH 85％),高温高湿创伤组(16只).采用Feeney自由落体法建立大鼠急性颅脑损伤模型.以上四组动物分别于6h、24h时间点断头处死,对脑组织含水量、HE染色后组织学观察、AQP4蛋白和mRNA的表达检测及电镜观察.结果 与常温创伤组比较,高温高湿创伤组AQP4&mRNA的表达和脑组织含水量明显增加(P＜0.01).结论 高温高湿环境能明显加重创伤性脑水肿.

Effect of high temperature and humidity environment on traumatic cerebral edema in rats

Objective To analyze the effect of high temperature(HT) and high humidity(HH) environment on traumatic cerebral edema(TCE) by studying the influence of HT and HH environment on cerebral water content and AQP4 expression in rats with cerebral injury.Methods Sixty-four healthy adult male SD rats were randomly divided into normal temperature(25℃) group without traumatic injury(n=16),normal humidity(RH 50%) group with traumatic injury(n=16),HT(35℃) and HH(RH 85%) group without traumatic injury(n=16),and HT(35℃) and HH(RH 85%)group with traumatic injury(n=16).An acute cerebral injury model of rats was established with the free-falling body method.6 and 24 hours after the rats in 4 groups were killed,cerebral water content and expression of AQP4 protein and mRNA were detected by electron microscopy with HE staining.Results The expression level of AQP4 and AQP4 mRNA and cerebral water content were significantly higher in normal temperature group with traumatic injury than in HT and HH groups with traumatic injury(P<0.01).Conclusion HT and HH environment can exacerbate TCE.