巴戟天对大鼠心肌缺血再灌注损伤的影响

目的 观察巴戟天(radix morindae officinalis,RMO)对大鼠心肌缺血再灌注损伤的影响并探讨其可能机制.方法 雄性大鼠40只,完全随机等分为假手术(sham operation,sham)组,心肌缺血/再灌注(myocardial ischemia/reperfusion,I/R)模型组,RMO分为高剂量组3.0g/(kg·d)、低剂量组1.2g/(kg·d),每组10只灌胃.Sham组和模型组给予等量蒸馏水灌胃,Sham组穿线不结扎,其余三组均穿线结扎冠状动脉左前降支(LAD)起始部30min,再灌注60min.观察心肌梗死面积、心肌组织中CK、NO、iNOS含量与Ca2+-Mg2+-ATPase、Na+-K+-ATPase活性的变化.结果 与模型组相比,RMO各剂量组均可明显缩小心肌梗死面积(P＜0.05),且心肌组织中Na+-K+-ATPase、Ca2+-Mg2+-ATPase、NO、iNOS活性升高,而CK含量降低(P＜0.05).结论 RMO有预防MIRI作用,其机制主要与防止钙超载及氧自由基有关.

Effect of Radix morindae offieinalis on myoeardial ischemia-reperfusion injury in rats

Objective To study the possible mechanism of radix morindae offieinalis(RMO) underlying myocardial ischemia-reperfusion injury(IRI) in rats by observing its effect on IRI.Methods Forty male rats were randomly divided into sham operation group,myocardial IRI model group,high-and low-dose RMO groups(10 rats in each group).Rats in sham operation group and high-and low-dose RMO groups received a same volume of distilled water.Coronary artery in rats of sham operation group was not ligated,while the left anterior descending(LAD) of coronary artery in rats of the other 3 groups was ligated for 30min and reperfused for 60min.Then,their cardiac Infarction size,and creatine kinase(CK),Na+-K+-ATPase,Ca2+-Mg2+-ATPase,nitric oxide(NO) and iNOS levels in myocardial tissue were measured.Results The cardiac infarction size was significantly smaller while the Na+-K+-ATPase,Ca2+-Mg2+-ATPase,NO and iNOS levels in myocardial tissue were higher and the CK level was lower in IRI model group than in high-and low-dose RMO groups(P<0.05).Conclusion RMO can protect myocardium against IRI in rats by preventing calcium overload and reducing oxygen free radicals.