| COPADG对食管癌裸鼠肿瘤增殖与细胞凋亡的影响及机制探讨 |
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| 引用本文： | 张晶,吴静,王爱勤.COPADG对食管癌裸鼠肿瘤增殖与细胞凋亡的影响及机制探讨[J].山东医药,2010,50(18):7-9. |
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| 作者姓名： | 张晶 吴静 王爱勤 |
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| 作者单位： | 1. 北京世纪坛医院,北京,100035
2. 中国科学院兰州化学物理研究所 |
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| 摘 要： | 目的观察COPADG对食管癌裸鼠肿瘤增殖与细胞凋亡的影响,并探讨其机制。方法建立人食管癌Eca-109裸鼠移植瘤模型并随机分为A、B、C、D四组,分别给予生理盐水、COPADG、5-FU、COPADG＋5-FU灌胃0.2ml,1次/d,连用28 d。测量动物体质量、肿瘤质量计算抑瘤率,采用免疫细胞化学法检测肿瘤组织中的Bcl-2、Caspase-3、PCNA。结果A、B、C、D组抑瘤率分别为0、54.16%、38.78%、73.91%,早期细胞凋亡率分别为6.02%、11.47%、13.05%、21.56%,晚期细胞凋亡率分别为12.65%、20.99%、13.23%、21.6%,B、C、D组与A组比较,P均〈0.01;D组抑瘤率与B、C组比较,P均〈0.01。与A组比较,B、C、D组Bcl-2、PCNA表达明显降低,Caspase-3表达明显增加（P均〈0.01）。结论COPADG可抑制食管癌裸鼠肿瘤增殖、诱导癌细胞凋亡,该作用可能与降低Bcl-2、PCNA表达以及增加Caspase-3表达有关。
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| 关 键 词： | D-氨基葡萄糖 食管肿瘤 B淋巴细胞瘤/白血病基因-2 天冬氨酸半胱氨酸特异性蛋白酶 增殖细胞核抗原 |
Effect and mechanism of COPADG on esophageal carcinoma cell roliferation and apoptosis in nude mice |
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| ZHANG Jing,WU Jing,WANG Ai-qin.Effect and mechanism of COPADG on esophageal carcinoma cell roliferation and apoptosis in nude mice[J].Shandong Medical Journal,2010,50(18):7-9. |
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| Authors: | ZHANG Jing WU Jing WANG Ai-qin |
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| Institution: | ZHANG Jing1,WU Jing,WANG Ai-qin(1Beijing Shijitan Hospital,Beijing100035,P.R.China) |
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| Abstract: | Objective To observe the effect and mechanism of multiplication and apoptosis of Hydroxy1-D-glucose(COPADG)on esophageal carcinoma cell poliferation and apoptosis in nude mice,and explore its mechanism.Methods The models of Eca-109 esophageal carcinoma in nude mice were established and divided into group A,B,C,D randomly.0.9%NaCl,COPADG,5-FU and COPADG+5-FU(CF)were used by intragastric administration in group A,B,C,D,0.2ml per day,28 days in total.The quality of animal and tumor were measured.The rate of su... |
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| Keywords: | D-glucose esophageal neoplasm B-cell lymphonma gene 2 aspartate-specific cysteine protease 3 proliferating cell nuclear antigen |
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