均匀设计优选玉红膏中紫草油炸提取工艺

目的:优选玉红膏中紫草的油炸提取工艺.方法:以羟基萘醌总色素得率为指标,通过单因素试验考察闷润溶剂种类、闷润溶剂浓度对羟基萘醌总色素的影响;紫外分光光度法测定羟基萘醌总色素含量,采用均匀设计法考察闷润溶剂浓度、闷润溶剂用量、油炸温度和油炸时间对提取工艺的影响,优选紫草的油炸提取工艺.结果:最佳油炸提取工艺为加3.20倍量68.87％乙醇闷润,油炸温度160.15℃,提取1.96 min.结论:采用均匀设计法优选的紫草油炸提取工艺稳定可行,为紫草的传统油炸提取工艺提供试验依据.

Optimization of Fried Extracting Process for Arnebia euchroma from Yuhong Ointment by Uniform Design

Objective:To optimize fried extraction process of Arnebia euchroma from Yuhong ointment.Method:With yield of hydroxyl naphthalene quinones total pigment as index,single factor test was performed to investigate kind of moistening solvent and the concentration of moistening solvent;UV-spectrophotometry was applied to determine the content of hydroxyl naphthalene quinones total pigment,fried extraction technology of A.euchroma was optimized by uniform design,which was used to investigate influence of moistening solvent concentration,solvent dosage,fired temperature and fired time on extraction process.Result:The best fried extraction technology was satisfied with some conditions as follows:moistened with 3.2 times the amount of 68.87% ethanol,fired temperature 160.15 ℃,fired time 1.96 min.Conclusion:This optimized extraction process was reasonable and stable,it could provide test basis for traditional fried extraction process of A.euchroma.