大孔吸附树脂法优选荔枝草总黄酮纯化工艺

目的:优选荔枝草总黄酮的富集纯化工艺.方法:以总黄酮吸附率、解吸率和转移率为评价指标,采用静态吸附-解吸附法,筛选大孔树脂型号;以总黄酮含量为指标,确定洗脱剂浓度;以除杂率、高车前苷转移率和总黄酮转移率为指标,优选其纯化工艺.结果:D101型树脂分离效果较好,其最佳工艺为生药量-树脂体积1 g·mL-1,用7 BV 75％乙醇溶液洗脱,洗脱流速6 mL·min-1.结论:D101树脂可有效地对荔枝草总黄酮进行富集纯化.

Optimization of Purification Technology for Total Flavonoids from Salvia plebeia by Macroporous Adsorption Resin

Objective:To optimize enrichment and purification technology of total flavonoids from Salvia plebeia. Method:With adsorption-desorption ratio and transfer ratio as indexes,optimum macroporous resin was selected by static adsorption-desorption ratio tests;The concentration of eluting agent was determined with the content of total flavonoids as index;Purification technology was optimized with impurity rate,transfer rate of homoplantaginin and transfer rate of total flavonoids as indexes. Result:D101 was selected for its excellent adsorption and desorption properties,optimum process was as follows:the amount of crude drugs to D101 macroporous resin 1 g·mL^-1,eluted with 7 BV 75% ethanol,elution flow rate 6 mL·min^-1. Conclusion:Total flavonoids from S. plebeia could be effectively purificated and separated by D101 macroporous resin.