产气肠杆菌EAM-Z1荚膜的去除对胞内pyNPase活力和菌体生长量的影响

研究产气肠杆菌(EAM-Z1)荚膜去除或变薄对于胞内嘧啶核苷磷酸化酶(PyNPase)活力的菌体生长量和影响。在培养基中增加碳源以去除荚膜或使荚膜变薄,研究其对菌体生长的影响,并通过转化合成5-氟尿苷的方法研究其对PyNPase活力的影响。结果:在培养基中增加4%的D-甘露糖,1%的甲醇,2%的甘油或2%的丙酮都能使产气肠杆菌的荚膜变薄;其中增加2%丙酮时,菌体量增加43.92%,在工业生产中有重要意义,转化率为57.5%,提高2.8%,PyNPase比活力提高4.8%,转化合成5-氟尿苷的最佳反应时间为45 min,缩短了1/4。

Effects of Thinning the Capsule of Enterobacter Aerogenes on the PyNPase Activity and the Growth of the Strain

The purpose is to get rid of or to thin the capsule of Enterobacter aerogenes(EAM-Z1),and to study the effects of thinning the capsule of EAM-Z1 on the growth of strain and the PyNPase activity.The capsule of EAM-Z1 was turned to be thinner by adding carbon source into medium.The effects of thinning the capsule on the PyNPase activity were studied by the conversion of 5-FUR and on the growth of EAM-Z1 and they were also studied by weighing thallus.Adding 4% D-mannose,1% methanol,2% glycerol or 2% acetone into medium can turning the capsule of EMA-Z1 thinner.The conversion rate reached 57.5% with 2.8% increased,the quantity of thallus was increased by 43.92% and the optimal time of reaction reached 45 minutes with a quarter shorten when adding 2% acetone into the medium.Adding 2% acetone to the medium could be applied to industrial production.