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| Array-CGH在甲醛固定石蜡包埋组织中的应用研究初探 | |
| 引用本文： | 李栋梁,尤伟艳,李永康,赵春霞,刘春霞,邹泓,刘旭伟,李锋. Array-CGH在甲醛固定石蜡包埋组织中的应用研究初探[J]. 农垦医学, 2011, 33(1): 14-18 |
| 作者姓名： | 李栋梁 尤伟艳 李永康 赵春霞 刘春霞 邹泓 刘旭伟 李锋 |
| 作者单位： | 1. 石河子大学医学院 2. 石河子大学医学院病理学教研室/新疆地方与民族高发病教育部重点实验室,新疆石河子,832002 3. 新疆生产建设兵团农六师医院病理科,新疆五家渠,831300 |
| 摘 要： | 目的：初步探讨微阵列比较基因组杂交技术在甲醛固定石蜡包埋组织中的应用及意义。方法：采用全基因组Array-CGH检测2例甲醛固定石蜡包埋横纹肌肉瘤组织的DNA拷贝数变化。结果：2例FFPE横纹肌肉瘤组织在全基因层面上均检测出不同程度的获得和缺失,同时检测出部分区段和基因的扩增和丢失：其中的1例ERMS扩增区段为2p22.3,2p24.3,8p22,8p12,8q12,8q24.13,8q24.22,12q13.3-14.1,22 q11.1-q11.23,丢失区段为15q25.2,16p12.3,16p13.2,17p11.2,4q13.2,6p21.31,6q25.3,9p23,21q22.13,22q11.23;另一例ARMS的扩增区段为1p21.1,1p36.3,2p11.1,2p24.3,2p22.3,5p14.3;丢失区段为2p12,3q26.1,3q29,9p23,与以往文献报道较为一致。结论：Array-CGH及其步骤的优化适于FFPE组织材料DNA拷贝数的检测,获得了与文献报道较为一致的检测结果。 |
| 关 键 词： | Array-CGH FFPE 肉瘤 比较基因组杂交 拷贝数改变 |
Cytogenetic analysis of formalin-fixed,paraffin-embedded tumor material by array-based comparative genomic hybridisation |
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| LI Dong-liang,YOU Wei-yang,LI Yong-kang,ZHAO Chun-xia,LIU Chun-xia,ZOU Hong,LIU Xun-wei,LI Feng. Cytogenetic analysis of formalin-fixed,paraffin-embedded tumor material by array-based comparative genomic hybridisation[J]. Agricultural Reclamation Medicine, 2011, 33(1): 14-18 | |
| Authors: | LI Dong-liang YOU Wei-yang LI Yong-kang ZHAO Chun-xia LIU Chun-xia ZOU Hong LIU Xun-wei LI Feng |
| Affiliation: | LI Dong-liang1,YOU Wei-yang1,LI Yong-kang1,ZHAO Chun-xia1,LIU Chun-xia1,ZOU Hong2,LIU Xun-wei3,LI Feng2(1.Class 2008 of Master of pathology,Shihezi Uniuersity school of Medicine,2.Department of Pathology,Shihezi University School of Medicine/Laboratory of Xinjiang Endemic and Ethnic Disease,Xinjiang Shehezi,832002,3.Xinjiang Production and Construction Crops,Nongliushi Hospital,Xingjiang Wujiaqu,831300) |
| Abstract: | Objective:To investigate and discuss array-based comparative genomic hybridization(array-CGH) Application in formalin-fixed,paraffin-embedded(FFPE) tumor material.Methods:Genomic DNA extracted FFPE tumour tissues was labelled with fluorochromes and then hybridised on to an array consisting of 720,000 probes representing regions throughout the entire human genome.Results:DNA copy number aberrations(CNAs) were found in whole genomic DNA of the 2 FFPE rhabdomyosarcoma(RMS).In the 1 embryonal RMS(ERMS),We found... |
| Keywords: | Array-CGH FFPE |
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