C57BL小鼠帕金森病模型多巴胺转运蛋白99mTc-TRODAT-1放射自显影研究

目的　评价不同损毁程度 Ｃ５７ Ｂ Ｌ 小鼠帕金森病（ Ｐ Ｄ）模型纹状体多巴胺转运蛋白（ Ｄ Ａ Ｔ）的变化。方法　根据腹腔注射 Ｍ Ｐ Ｔ Ｐ 的天数将小鼠分为 １、３、５ 和 ７ｄ 模型组以及对照组，静脉注射９９ｍ Ｔｃ Ｔ Ｒ Ｏ Ｄ Ａ Ｔ１６ｍ Ｃｉ，１ｈ 后处死行脑纹状体放射自显影，同时行免疫组化酪氨酸羟化酶（ Ｔ Ｈ）染色。结果　对照组的放射自显影可见９９ｍ Ｔｃ Ｔ Ｒ Ｏ Ｄ Ａ Ｔ１ 于纹状体部位有高度放射性聚集，且两侧纹状体基本相同。注射 Ｍ Ｐ Ｔ Ｐ １ｄ 者，其纹状体的放射性浓集比对照组有所下降。注射 Ｍ Ｐ Ｔ Ｐ ３、５ 及 ７ｄ 者，两侧纹状体的放射性浓集逐日降低，第 ７ｄ 者几乎消失。 Ｔ Ｈ 染色发现黑质 Ｔ Ｈ 阳性神经元亦随注射 Ｍ Ｐ Ｔ Ｐ 天数的增加而数量减少。结论　不同程度损毁的 Ｃ５７ Ｂ Ｌ 小鼠 Ｐ Ｄ 模型可模拟 Ｐ Ｄ 的发展过程，９９ｍ Ｔｃ Ｔ Ｒ Ｏ Ｄ Ａ Ｔ１ 作为 Ｄ Ａ Ｔ 的显影剂可用于早期诊断的神经显像学研究。

Autoradiographic study on dopamine transporter in C57BL mouse models of Parkinsonism with 99mTc-TRODAT-1

Objective To evaluate the density change of dopamine transporter(DAT)in striatum of C57BL mouse model of Parkinsonism.Methods In this study,MPTP and Tc TRODAT 1 were injected intravenously to mice respectly in several groups.Autoradiography and histoimmunochemical stain technology were used respectly.Result At 1h postinjection of 99m Tc TRODAT 1,exvivo autoradiography indicated that the striatum displayed the intense radioactivity in the control group.In group of MPTP lesion for 1 day,the radioactivity of the striatum was reduced compared with that of control group.In groups of 3,5 and 7 days,the radioactivity of the striatum was decreased,and the degree of decrement was related positive to the days of MPTP lesion,and in group of 7 days,the radioactivity in striatium almost disappeared.The loss of TH positive cells and fibres in substantia nigra and striatum of models were found,and the degree of loss was also related positive to the days with MPTP lesion.Conclusion The progressive degeneration of Parkinson ,s disease was mimicked by C57BL mouse model of parkinsonism with the different degree of MPTP lesioned substantia nigra. 99m Tc TRODAT 1 as a imaging ligand for DAT may be useful for the early diagnosis of PD.