| 电融合技术与半固体培养技术结合制备抗人肠道病毒单… |
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| 关 键 词： | 电融合 半固体培养 肠道腺病毒 单克隆抗体 制备 |
Production of anti-enteric adenovirus monoclonal antibody by electrofusion and semi-solid medium culture] |
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| Y Zhang,L Chen,S Wang,C Guo,P Mao. Production of anti-enteric adenovirus monoclonal antibody by electrofusion and semi-solid medium culture][J]. Chinese journal of experimental and clinical virology, 1997, 11(1): 69-71 |
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| Authors: | Y Zhang L Chen S Wang C Guo P Mao |
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| Affiliation: | Institute of Basic Medical Science, Chinese Academy of Medical Sciences, Beijing 100005. |
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| Abstract: | Ten million splenocytes from the enteric adenovirus (Ad40/41) immunized mice were fused with 3 x 10(6) myeloma cells by electrofusion. Then, the cells were selectively cultured in methyl cellulose semi-solid medium. Eighteen clones of hybridoma were directely picked out from the culture plates and 7 of them could secret high level specific antibodies to enteric adenovirus. So far, the anti-Ad40/41 virus monoclonal antibodies were successfully generated by the protocal of electrofusion accompanied with selective culture in methyl cellulose semi-solid medium. |
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