乙型肝炎病毒前S2抗原／抗体检测方法的改进及其应用

通过改进乙型肝炎病毒表面前Ｓ２抗原（Ｐｒｅ－Ｓ２Ａｇ）、前Ｓ２抗体（Ｐｒｅ－Ｓ２Ａｂ）的检测方法,对１２８份正常人标本Ｐｒｅ－Ｓ２Ａｂ进行测定,确定了Ｐｒｅ－Ｓ２Ａｂ抑制率的阳性界值。用改进后的方法检测不同人群中的Ｐｒｅ－Ｓ２Ａｇ及Ｐｒｅ－Ｓ２Ａｂ,发现在慢性乙型肝炎患者中Ｐｒｅ－Ｓ２Ａｇ比ＨＢｅＡｇ／Ａｂ系统更能反映机体的ＨＢＶＤＮＡ复制水平（Ｐ＜０．０１）,而Ｐｒｅ－Ｓ２Ａｂ的检出则与急性乙型肝炎的预后密切相关,同时,Ｐｒｅ－Ｓ２Ａｂ也可作为评价含有Ｐｒｅ－Ｓ２Ａｇ的乙肝疫苗主动免疫效果的指标。

Improvement of the assay for Pre S2 Ag/Ab detection and its application in different patients

A new ELISA inhibition test for measuring serum Pre-S2Ab was developed to avoid the "double positive" phenomenon. One hundred and twenty samples with Pre-S2Ab negative were detected for Pre-S2Ab, the critical range for Pre-S2Ab in normal population was confirmed: a positive result was decided while the inhibition ration was over 50%, a negative result was decided while the inhibition ratio was less than 34%, and a dubious result was decided while the ratio was over 34% but less then 50%. At the same time, serum samples from sixty patients with chronic hepatitis B were detected for Pre-S2Ag and Pre-S2Ab, it showed that (1) the positive ratio of HBV DNA in patients with Pre-S2Ag positive was much higher than in patients with Pre-S2Ag negative (P < 0.01); (2) to the 46 patients with HBV DNA positive, Pre-S2Ag had a better relationship with HBV DNA than the HBeAg/Ab did (P < 0.01). Furthermore, to patients with acute hepatitis B, the presence of Pre-S2Ab was closely related to the prognosis of the disease, and the Pre-S2Ab assay could also be used to evaluate the preventive effect of HB vaccine containing Pre-S2Ag.