从人群中分离到1株A／PR／8／34（H1N1）类病毒

１９９１年５月从１名上呼吸道感染的儿童患者中分离出１株流感病毒。经血清学鉴定和分析，分离物为Ａ／ＰＲ／８／３４（Ｈ１Ｎ１）类似毒株。病毒ＨＡ１区基因经核苷酸序列分析，分离物与Ａ／ＰＲ／８／３４毒株间有１２个位点不同，导致了ＨＡ１区蛋白分子上４个氨基酸的替换。在病毒基因组寡核苷酸指纹图分析中发现，分离物与Ａ／ＰＲ／８／３４毒株相比较丢失了２个点，而插入了９个点。因此，Ａ／广东／６／９１（Ｈ１Ｎ１）毒株不是由Ａ／ＰＲ／８／３４（Ｈ１Ｎ１）通过实验室污染而来。

A strain of A/PR/8/34 (H1N1)-like virus isolated from men

An influenza virus was isolated in May, 1991 from a child with acute respiratory disease. The identification results with serologic method have revealed that the isolate was A/PR/8/34 (H1N1)-like virus. The analyses of nucleotide sequences in HA1 domain showed that there were 12 different positions on nucleotide sequences between the isolate and the A/PR/8/34 (H1N1) virus. These differences induced substitutions of 4 amino acids in HA1 protein molecule of isolate as compared with that of A/PR/8/34 (H1N1) virus. The oligonucleotide analyses of viral genome revealed that there were 11 different points between isolate and A/PR/8/34 (H1N1) virus, e. g. 2 deleted and 9 inserted in genome of isolate as compared with A/PR/8/34 (H1N1) strain. Therefore, the isolate possibly derived from A/PR/8/34 (H1N1) virus by laboratory contamination could be ruled out. However, where did A/Guangdong/6/91 (H1N1) virus come from? This question will be settled by further study.