CAP系统检测过敏原特异性IgE抗体的方法学评价

目的 评价CAP过敏原检测系统检测过敏原特异性IgE抗体的准确性和预测值。方法 研究对象为2003～2004年就诊的病例，结合病史和体内试验结果做出过敏原特异性的临床诊断。对其中明确诊断为尘螨、蒿属花粉、律草花粉、猫毛屑或链格孢霉5种过敏原过敏者用CAP过敏原检测系统进行相应的过敏原特异性IgE检测，并随机选取部分临床明确诊断对上述5种过敏原不过敏者同时进行相应过敏原特异性IgE检测，分析CAP系统检测的准确性并计算其阳性预测值和阴性预测值。结果 共进行了957项次过敏原特异性IgE检测，对尘螨、蒿属花粉、律草花粉、猫毛屑、链格孢霉特异性IgE检测的灵敏度分别为99．0％、97．9％、91．7％、93．5％、85．5％，特异性分别为96．2％、95．8％、93．0％、97.0％、91．7％。特异性IgE检测结果与临床诊断之间的符合率较高，阳性预测值均大于94％，阴性预测值均大于80％。结论 用CAP过敏原检测系统检测过敏原特异性IgE抗体的结果准确性好，预测值高，表明该方法具有较高的实用诊断价值。

Evaluation of CAP test system for allergen-specific IgE assay

Objective To evaluate the validity and predictive value of CAP specific IgE test system. Methods Five allergen-specific IgEs, i.e. , specific IgE to mites, mugwort pollens, hop pollens,cat epithelium and dander, and Alternaria alternate,were tested in selected randomly allergic and non-allergic patients by using CAP system. Results In 957 tests the sensitivity of specific IgE to the 5 allergens was 99.0% ,97.9% ,91.7% ,93.5% and 85.5% ,and the specificity was 96.2% ,95.8% ,93.0% ,97.0% and 91.7%. The positive and negative predictive values were greater than 94% and 80% respectively. Conclusion CAP system is of great potential value for diagnosis of allergenas.