| IL-18及其受体在特发性血小板减少性紫癜患者Th1类细胞优势应答中的作用 |
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| 引用本文： | 王谦,杨晓静,单宁宁,侯明,冀学斌,王春燕,朱效娟,杨蕾,张晓琳,彭军,马道新,石艳.IL-18及其受体在特发性血小板减少性紫癜患者Th1类细胞优势应答中的作用[J].中华血液学杂志,2009,30(10). |
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| 作者姓名： | 王谦 杨晓静 单宁宁 侯明 冀学斌 王春燕 朱效娟 杨蕾 张晓琳 彭军 马道新 石艳 |
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| 作者单位： | 1. 山东大学齐鲁医院检验科,济南,250012
2. 山东大学齐鲁医院血液科,济南,250012 |
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| 基金项目： | 国家973资助项目，国家自然科学基金，国家卫生行业公益性科研项目，卫生部临床学科重点项目，教育部全国优秀博士论文专项基金资助项目，山东省中青年科学家科研奖励基金，山东省自然科学基金 |
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| 摘 要： | 目的 探讨白细胞介素18(IL-18)及其受体(IL-18R)在特发性血小板减少性紫癜(ITP)患者Th1类细胞因子优势应答中的作用.方法 应用逆转录聚合酶链反应(RT-PCR)检测15例活动期和18例缓解期ITP患者外周血单个核细胞(PBMNC)中转录因子T-bet和GATA-3 mRNA的表达;应用ELISA法检测血浆中IL-18的变化;应用流式细胞术检测CD3+细胞和淋巴细胞表面IL-18R的表达.13名健康志愿者为正常对照.结果 ITP活动期患者PBMNC中T-bet mRNA表达水平(0.069±0.013)明显高于对照组(0.019±0.010)(P＜0.05),而GATA-3 mRNA的表达水平(0.002±0.001)明显低于对照组(0.005±0.002)(P＜0.05);血浆IL-18和CD3+细胞表面IL-18R表达水平较ITP缓解期患者和对照组显著增高.ITP缓解期患者T-bet与GATA-3 mRNA表达水平与对照组比较差异均无统计学意义(P值均＞0.05),T-bet/GATA-3比例基本恢复正常,血浆中IL-18和CD3+细胞表面IL-18R的表达水平也基本恢复正常.结论 ITP活动期患者表现为Th1优势应答,T-bet/GATA-3比例明显失衡,T-bet/GATA-3比例可作为ITP患者Th1类细胞极化的敏感指标;ITP活动期患者IL-18和IL-18R表达上调,可能为ITP患者的治疗提供一个新的治疗靶点.
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| 关 键 词： | 紫癜 血小板减少性 特发性 转录因子T-bet 转录因子GATA-3 白细胞介素18 受体 白细胞介素18 |
The role of interleukin-18 and interleukin-18 receptor in predominant Th1 immune response of patients with immune thrombocytopenia |
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| Abstract: | Objective To evaluate the role of interleukin(IL)-18 and IL-18 receptor(IL-18R)in the predominant Thl type cytokine response in patients with immune thrombocytopenia(ITP).Methods Fifteen patients with active phase ITP,eighteen in remission and thirteen healthy controls were enrolled in this study.T-bet and GATA-3 mRNA levels in peripheral blood mononueleated cells(PBMNC)were measured by reverse transcfiptase polymerase chain reaction(RT-PCR);the plasma IL-18 level by enzyme linked immunosorbent assay(ELISA),the expression of IL-18R on CD3+ lymphocytes and total lymphocytes by flow cytometry(FCM).Results The T-bet mRNA levels in patients with active phase ITP was 3.572 fold as much as that in the controls(P＜0.05),while the GATA-3 mRNA levels were 0.378 foId of that in controls(P＜O.05).The levels of plasma IL-18 and IL-18R on CD3+ lymphocytes were significantly increased in active ph8se ITP than in remission phase and controls.There was no differenee in ratio of T-bet/GATA-3 between remitted ITP and controls and so was for T-bet mRNA,GATA-3 mRNA,plasma IL-18 and IL-18R on CD3+lymphocytes.Conclusion ITP as a disease of Thl-dominant response there is an unbalance between T-bet and GATA-3 in its active phase:IL-18 and IL-18R being upregulated. |
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