细胞凋亡抑制基因bcl-2在牙源性病损中表达的研究

目的　观察凋亡相关基因bcl- 2在成釉细胞瘤 (Ameloblastoma,AB)和牙源性角化囊肿 (OdontogenicKeratocyst,OKC)中的表达 ,探讨其与AB和OKC的生物学意义。方法　应用免疫组化法 (S -P)检测 75例AB(原发 31例 ,复发 37例 ,恶变 7例 ) ,35例OKC及 9例口腔正常粘膜。同时采用原位杂交法检测了 2 0例AB(12例原发 ,8例复发 ) ,12例OKC中的bcl- 2mRNA。结果　 88% (6 6 / 75 )AB ,74.2 % (2 6 / 35 )OKC ,44 .4% (4 / 9)正常口腔粘膜有bcl- 2蛋白表达 ,三组间相比差异有显著性 (P <0 .0 0 1)。同样在原发组AB 77.4% (2 4/ 31) ,复发组AB94.5 % (35 / 37) ,恶性组AB10 0 % (7/ 7)。bcl- 2阳性率及阳性强度随着组织学分级变化而增加 ,组间统计 (P <0 .0 5 )。在 2 0例AB及 12例OKC中bcl- 2mRNA表达趋势与bcl- 2蛋白的表达是相一致的 (P <0 .0 1)。bcl- 2阳性表达与AB不同组织学分型 ,病损发生部位 ,肿瘤生长方式 (单房与多房 )无关 (P >0 .0 5 )。bcl- 2在病变中表达的特征为AB的外周层细胞为强表达 ,星网状层有阳性表达 ,随着细胞增殖分化呈实性片状时bcl- 2表达增强 ,角化退变样细胞 ,颗粒性变细胞表达缺失。OKC中多在基底层细胞中表达。结论　①bcl- 2在AB及OKC的发生、发展及细胞分化与增殖中起着重要作用。

Investingation of bcl-2 gene expression in human odontogenic lesions

Objective To study bcl-2 expression in ameloblastoma(AB) and odontogenic keratocyst(OKC) and to investigate biological behaviour of AB and OKC.Methods Bcl-2 protein in 75 cases of AB(primary AB 31 cases,recurrent AB 37 cases,malignant AB 7 cases),35 cases of OKC and 9 cases of oral normal mucosa was detected by S-P method.Bcl-2 mRNA in 20cases of AB(primary AB 12 cases,recurrent AB 8 cases)and 12 cases of OKC was detected by in situ hybridization.Results Bcl-2 protein was expressed in 88% AB(66/75), 74.2% OKC (26/35) and 44.4% oral normal mucosa(4/9)(P< 0.001).The positive rate of primary,recurrent,malignat AB was 77.4%(24/31), 94.5%(35/37),100%(7/7),respectively (P< 0.05).Bcl-2 mRNA expression was correlated with bcl-2 protein in AB and OKC.There was no statistical difference between bcl-2 expression and pathology in AB.Conclusion Bcl-2 plays an importmant role in genesis and development of AB and OKC and is related with cell differentiation and proliferation.