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Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia |
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| Authors: | Osamu Marukawa Yukihiro Akao Johji Inazawa Takeshi Ariyama Tatsuo Abe Tomoki Naoe Mitsune Tanimoto Hidehiko Saito Yoshinori Otsuki & Yoshihide Tsujimoto |
| Institution: | Department of Anatomy and Biology, Osaka Medical College, Takatsuki, Osaka,;Department of Hygiene, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto,;Department of Internal Medicine, Nagoya University Branch Hospital, Nagoya, Aichi,;Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, Aichi,;Biomedical Research Centre, Osaka University Medical School, Suita, Osaka, Japan |
| Abstract: | Southern blot analysis with a cDNA probe of MLL indicated that the breakpoint is in a Bam HI 8.3 kb fragment which carries the exon 5–11 of MLL gene in DNA from an adult acute myelomonocytic leukaemia with a t(11;22)(q23;q11) translocation. The structural analysis of the rearranged MLL locus demonstrated that the breakpoint is localized between exon 8 and 9 of MLL locus. The normal counterpart fused to the MLL locus was proved to be derived from chromosome 22q11( AF-22 ) by somatic cell hybrids analysis and FISH. By FISH, AF-22 locus was localized to the region more centromeric to the BCR gene. |
| Keywords: | acute myelomonocytic leukaemia 11q23 t(11 22)(q23 q11) MLL AF-22 |