| 抗恶性疟原虫Fcc7801／HN株单克隆抗体的制备 |
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| 引用本文： | 蒋春雷 王笑利. 抗恶性疟原虫Fcc7801／HN株单克隆抗体的制备[J]. 第二军医大学学报, 1992, 13(3): 221-224 |
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| 作者姓名： | 蒋春雷 王笑利 |
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| 作者单位： | 第二军医大学训练部寄生虫学教研室 200433(蒋春雷，管惟滨)，长征医院临床免疫中心 200003(王笑利)，长征医院临床免疫中心 200003(孔宪涛) |
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| 摘 要： |
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| 关 键 词： | 恶性疟原虫 单克隆抗体 免疫学 |
Establishment of the Monoclonal Antibodies against Plasmodium falciparum Fcc7801/HN |
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| Jiang Chunlei,Guan Weibin,Wang Xiaoli,Kong Xiantao. Establishment of the Monoclonal Antibodies against Plasmodium falciparum Fcc7801/HN[J]. Former Academic Journal of Second Military Medical University, 1992, 13(3): 221-224 |
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| Authors: | Jiang Chunlei Guan Weibin Wang Xiaoli Kong Xiantao |
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| Abstract: | Four cell lines (C6, H6, B6, 2H6) of hybrids secreting monoclonal antibodies (McAb) against erythrocytic stage of Plasmodium falciparum Fcc7801/HN were established by fusing SP2/0 myeloma cells with spleen cells of BALB/c mice immuniaed with the preparation of the same malaria parasite and cloning three times. Of these four lines, C6 and B6 showed significant growth-inhibition of P. falciparum in vitro. Both C6 and B6 McAb recognized 71 kD antigen of P. falciparum by Western Blotting. The results indicate that the 71 kD antigen is of immune protection The four McAb showed cross immunofluorescence reactivity with P. falcipanum Fcc-1/HN, Fcc7802/HN, Fcc8703/JS and P. berghei. P. cynomolgi. It indicates that there are common epitopes among these malaria parasites, and that it is important in immunologic research and serodiagnosis of malaria |
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| Keywords: | Plasrnodium falciparum antibodies monoclonal immunoblotting fluorescent antibody tedmic |
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